蝉花液态培养菌丝体对高葡萄糖膳食LY品系白猪之亚急毒性探讨

A Subacute Toxicity Study of Cordyceps cicadae Mycelium in High-Glucose Diet-Fed LY Pigs

摘要: 蝉花为名贵传统中药材，具有保护眼睛、保护肾功能等多样生理功效，其菌丝体及子实体可以人工培养方式大量生产。本篇研究以采集自台湾新北市山区的野生大蝉花作为菌种来源，并以液态发酵方式培养蝉花菌丝体，进行LY品系白猪28天重复剂量安全性评估试验。本实验设对照组(添加60克葡萄糖至每日猪只饲粮中)及蝉花低、高两个剂量，即添加20克或40克的蝉花及60克的葡萄糖至每日猪只饲粮中。每个组别3只猪，连续喂养28天，每日进行实验动物之临床观察，且于试验开始及结束时测量猪只体重。28天后取猪只血液进行血液学、生化学检查，大体解剖观察有无明显病变并进行组织病理学检查。试验结果显示，试验期间所有受试动物生长情况良好，无明显临床症状差异发生，血液学检查、生化学检查及组织病理学检查结果与对照组相比，亦均无明显差异。

Abstract: Cordyceps cicadae (C. cicadae) is a valued traditional Chinese medicine. C. cicadae exhibits a variety of biological functions, including vision improvement and protection of renal function. Because of the shortage of wild C. cicadae, artificial cultivation is an ideal substitute. The present study was conducted to investigate the 28-day oral toxicity of C. cicadae in LY pig. The C. cicadae sample was collected from the mountains of New Taipei City and mass produced by submerged cultivation. Nine pigs were divided into three groups and were fed with 0, 20 or 40 g of freeze dried C. cicadae powder in combination with 60 g glucose for 28 consecutive days. Clinical observation of the pigs was carried out daily. The body weights of the pigs were recorded at the beginning and the end of the study. At the end of the study, all pigs were sacrificed and the blood and organs were collected for hematology, clinical biochemistry and histopathological examination. During the study period, no abnormality occurred in clinical signs and body weight. There was no significant difference in hematology and clinical biochemistry parameters between the treatment and control group. Ne-cropsy and histopathological examination showed no treatment-related change.