尼罗罗非鱼皮胶原蛋白乳酸提取工艺条件优化及理化特性研究
Collagen Extraction from Nile Tilapia (Oreochromis niloticus) Skin: Optimization of Extraction Conditions Using Response Surface Methodology and Physicochemical Characterization
DOI: 10.12677/HJFNS.2016.52008, PDF,  被引量   
作者: 梁志桃, 郭 飞, 胡柳花, 鹿文红：上海格瑞产品检测有限公司，上海 ；农业部设施园艺产品质量安全控制重点实验室，上海 ；张强：上海格瑞产品检测有限公司，上海 ；农业部设施园艺产品质量安全控制重点实验室，上海 ；武汉大学基础医学院，湖北 武汉
关键词: 罗非鱼皮；乳酸；胶原蛋白；响应面分析法；理化特性；Tilapia Skin； Lactic Acid； Collagen； Response Surface Methodology； Physicochemical Property
摘要: 以罗非鱼皮为原料，乳酸为介质提取胶原蛋白。通过单因素实验分别考察pH值、液固比和提取时间对胶原蛋白提取率的影响，在此基础上，利用Box-Behnken中心组合实验设计和响应面分析法对乳酸提取胶原蛋白条件进行优化，并对胶原蛋白的理化性质进行评估。结果表明：乳酸提取罗非鱼皮胶原蛋白(ASC)的优化工艺条件为pH2.1、液固比65:1 (mL/g)、时间44 h，在此条件下罗非鱼皮胶原蛋白提取率可达85.57%，ASC的亚基组成形式为(α1)2α2，热变性温度为32.56℃，呈现均匀的纤维结构。
Abstract: This study was undertaken to optimize the acidic extraction of collagen from Nile tilapia (Oreoch-romis niloticus) skin. Lactic acid was used to extract collagen. Further, three process conditions affecting the yield of collagen extraction with lactic acid, such as pH value, liquid-to-solid ratio and extraction time, were optimized by single factor experiment. According to this, Box-Behnken ex-perimental design combined with response surface methodology was taken to optimize the ex-tracting conditions of collagen using lactic acid. Based on the optimized conditions, physicochemical properties of collagen were evaluated. The results indicated that the optimal conditions for collagen extraction were pH2.1, liquid-to-solid ratio 65:1 (mL/g) and extraction time 44 h, under which a yield of collagen of 85.57% was obtained. The ASC showed that SDS-PAGE pattern, the thermal denaturation temperature, and morphology were (α1)2α2, 32.56℃, and evenly fibrous structure, respectively.
文章引用：梁志桃, 郭飞, 胡柳花, 鹿文红, 张强. 尼罗罗非鱼皮胶原蛋白乳酸提取工艺条件优化及理化特性研究[J]. 食品与营养科学, 2016, 5(2): 52-62. https://dx.doi.org/10.12677/HJFNS.2016.52008

参考文献

[1] Pati, F., Adhikari, B. and Dhara, S. (2010) Isolation and Characterization of Fish Scale Collagen of Higher Thermal Stability. Bioresource Technology, 101, 3737-3742. http://dx.doi.org/10.1016/j.biortech.2009.12.133 [Google Scholar] [CrossRef] [PubMed]
[2] Matmaroh, K., Benjakul, S., Prodpran, T., Encarnacion, A.B. and Kishimura, H. (2011) Characteristics of Acid Soluble Collagen and Pepsin Soluble Collagen from Scale of Spotted Golden Goatfish (Parupeneus heptacanthus). Food Chemistry, 129, 1179-1186. http://dx.doi.org/10.1016/j.foodchem.2011.05.099 [Google Scholar] [CrossRef] [PubMed]
[3] Zhang, J.J., Duan, R., Tian, Y.Y. and Konno, K. (2009) Characterisation of Acid-Soluble Collagen from Skin of Silver Carp (Hypophthalmichthys molitrix). Food Chemistry, 116, 318-322. http://dx.doi.org/10.1016/j.foodchem.2009.02.053 [Google Scholar] [CrossRef] 
[4] Kittiphattanabawon, P., Benjakul, S., Visessanguan, W. and Shahidi, F. (2010) Isolation and Properties of Acid- and Pepsin-Soluble Collagen from the Skin of Blacktip Shark (Carcharhinus limbatus). European Food Research and Technology, 230, 475-483. http://dx.doi.org/10.1007/s00217-009-1191-0 [Google Scholar] [CrossRef] 
[5] Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Nagai, T. and Tanaka, M. (2005) Characterisation of Acid- Soluble Collagen from Skin and Bone of Bigeye Snapper (Pria-canthus tayenus). Food Chemistry, 89, 363-372. http://dx.doi.org/10.1016/j.foodchem.2004.02.042 [Google Scholar] [CrossRef] 
[6] Liu, D.S., Liang, L., Regenstein, J.M. and Zhou, P. (2012) Extraction and Characterisation of Pepsin-Solubilised Collagen from Fins, Scales, Skins, Bones and Swim Bladders of Bighead Carp (Hypophthalmichthys nobilis). Food Chemistry, 133, 1441-1448. http://dx.doi.org/10.1016/j.foodchem.2012.02.032 [Google Scholar] [CrossRef] 
[7] Zeng, S.K., Zhang, C.H., Lin, H., et al. (2009) Isolation and Characterisation of Acid-Solubilised Collagen from the Skin of Nile Tilapia (Oreochromis niloticus). Food Chemistry, 116, 879-883. http://dx.doi.org/10.1016/j.foodchem.2009.03.038 [Google Scholar] [CrossRef] 
[8] 叶小燕, 曾少葵, 余文国, 吴文龙, 曾祥根, 黄丽明. 罗非鱼皮营养成分分析及鱼皮明胶提取工艺的探讨[J]. 南方水产, 2008, 4(5): 55-60.
[9] Baş, D. and Boyaci, İ.H. (2007) Modeling and Optimization I: Usability of Response Surface Methodology. Journal of Food Engineering, 78, 836-845. http://dx.doi.org/10.1016/j.jfoodeng.2005.11.024 [Google Scholar] [CrossRef] 
[10] 吴祖兴. 现代食品生产[M]. 北京: 中国农业大学出版社, 2000: 238.
[11] 关静, 叶萍, 武继民. 胶原海绵的羟脯氨酸含量测定[J]. 氨基酸和生物资源, 2000, 22(1): 52-54.
[12] Zhang, Y., Liu, W.T., Li, G.Y., et al. (2007) Isolation and Partial Characterization of Pepsin-Soluble Collagen from the Skin of Grass Carp (Clenopharyngodon idella). Food Chemistry, 103, 906-912. http://dx.doi.org/10.1016/j.foodchem.2006.09.053 [Google Scholar] [CrossRef] 
[13] Zhang, B., Chen, Y.Z., Wei, X.F., et al. (2010) Optimiza-tion of Conditions for Collagen Extraction from the Swim Bladders of Grass Carp (Ctenopharyngodon idella) by Re-sponse Surface Methodology. International Journal of Food Engineering, 6, 1556-1772. http://dx.doi.org/10.2202/1556-3758.1772 [Google Scholar] [CrossRef] 
[14] 王镜岩. 生物化学[M]. 第三版. 北京: 高等教育出版社, 2002: 106-108.
[15] 杨霞, 王珊珊, 赵芙钗, 陶宇, 李八方. 驴皮中胶原蛋白的提取及其特性[J]. 精细化工, 2011, 28(9): 884-886.
[16] Dedirmenbasi, N., Kalyon, D.M. and Birinci, E. (2006) Biocomposites of Nanohydroxyapatite with Collagen and Poly(vinyl alcohol). Colloid and Surface B, 48, 42-49. http://dx.doi.org/10.1016/j.colsurfb.2006.01.002 [Google Scholar] [CrossRef] [PubMed]
[17] Tamilmozhi, S., Veeruraj, A. and Arumugam, M. (2013) Isolation and Characterization of Acid and Pepsin-Solubilized Collagen from the Skin of Sailfish (Istiophorus platyp-terus). Food Research International, 54, 1499-1505. http://dx.doi.org/10.1016/j.foodres.2013.10.002 [Google Scholar] [CrossRef]