白藜芦醇对小鼠非酒精性脂肪肝的预防作用研究
Protective Effect of Resveratrol against Non-Alcoholic Fatty Liver Disease in Mice
DOI: 10.12677/HJFNS.2018.71003, PDF,    国家自然科学基金支持
作者: 毛思宇：河北农业大学生命科学学院，河北 保定；湖南农业大学动物科学技术学院，湖南 长沙；陈 杰, 伍小松, 苏丁丁：湖南农业大学动物科学技术学院，湖南 长沙；杨 青：湖南农业大学动物医学院，湖南 长沙
关键词: 白藜芦醇；小鼠；非酒精性脂肪肝；预防作用；Resveratrol； Mouse； Non-Alcoholic Fatty Liver Disease； Protective Effect
摘要: 本研究使用高脂日粮饲喂小鼠建立NAFLD动物模型，研究白藜芦醇(Resveratrol, RES)对非酒精性脂肪肝(Non-alcoholic fatty liver disease, NAFLD)的预防作用效果。采用H.E.染色、比色法及Luminex多因子检测等方法检测了RES干预处理后对体重、肝脏组织学变化、肝脂和血脂成分以及血清中细胞因子等的变化。结果显示：小鼠饲喂高脂日粮诱导后，体重在第4~5周时显著高于正常日粮饲喂对照组(p ˂ 0.05)；肝脏组织受到明显的病理学损伤；血脂和肝脂包括甘油三酯(Triglyceride, TG)、总胆固醇(Total cholesterol, TC)、低密度脂蛋白胆固醇(Low-density lipoprotein cholesterol, LDL-C)的水平均显著高于空白对照组，而高密度脂蛋白胆固醇(High-density lipoprotein cholesterol, LDL-C)则降低；血清转氨酶活力包括丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)、血清中细胞因子白介素(Interleukin, IL)-2、IL-6、IL-1β以及肿瘤坏死因子(Tumor necrosis factor alpha, TNF-α)的水平显著升高。用RES干预处理后，高脂所诱导的脂肪肝病变明显减轻，并在不同程度上对血脂和肝脂成分、血清转氨酶及部分细胞因子水平起到相应的调节作用。以上结果揭示，RES对小鼠NAFLD的发生有一定的预防作用。
Abstract: To investigate the protective effect of resveratrol (RES) on non-alcoholic fatty liver disease (NAFLD) in mice, in the present study, a NAFLD mouse model was established through feeding high-fat diet. Changes in body weight, liver histopathology, lipid composition of liver and serum, and seral cytokines were studied after interventive treatment with RES in the NAFLD mice by us-ing H.E. staining, colorimetric method and Luminex test for multi-factor. The results showed that after feeding high-fat diet, the mouse body weight was higher than that of the normal control group between 4~5 weeks (p ˂ 0.05); histopathological injury in liver tissue was obvious; lipid components in liver and serum including triglyceride (TG), total cholesterol (TC), low density li-poprotein cholesterol (LDL-C) levels were significantly increased, and the high density lipoprotein cholesterol (LDL-C) was significantly decreased; seral transaminases including ALT and AST, seral interleukin (IL)-2, IL-6, IL-1 beta and tumor necrosis factor (TNF-α) were also significantly increased. After intervention with RES, fatty liver lesions induced by high-fat diet were significantly alleviated; and levels of lipid components in liver and serum, seral transferase and cytokines were regulated by RES to some extent. All data indicate that RES has a preventive effect against the NAFLD in mice.
文章引用：毛思宇, 陈杰, 杨青, 伍小松, 苏丁丁. 白藜芦醇对小鼠非酒精性脂肪肝的预防作用研究[J]. 食品与营养科学, 2018, 7(1): 17-25. https://doi.org/10.12677/HJFNS.2018.71003

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