褐藻胶裂解酶提高海带降解效果的应用研究

Application Study on Alginate Lyase Enhancing the Degradation Effect of Kelp

摘要: 本研究以干海带为原料，通过褐藻胶裂解酶、复合酶、褐藻胶裂解酶 + 复合酶、未加酶四组实验，对海带降解的降粘效果、总糖含量、还原糖含量、酶解得率、营养成分进行对比分析，探究褐藻胶裂解酶对海带降解的促进作用。结果表明褐藻胶裂解酶 + 复合酶组的海带降解效果最佳，海带酶解得率分别比褐藻胶裂解酶组、复合酶组和未加酶组提高53.2%、174.5%和245.1%，且Na⁺、Mg²⁺对海带酶解得率具有促进作用，最终获得的海带酶解液的初始干基可达61.3 g/L，总糖、还原糖、有机质及多种矿物质含量也有提高。

Abstract: In this study, dry kelp was as raw material to explore the catalytic effect on alginate lyase enhancing the degradation of kelp. Four groups of experiments, including alginate lyase, complex enzyme, alginate lyase-complex enzyme and no enzyme, were designed. Viscosity reducing effect, total sugar content, reducing sugar content, enzymatic hydrolysis yield and the nutritional components were compared and analyzed. The results showed that kelp degradation effect of alginate lyase-complex enzyme was the best. Compared with alginate lyase group, compound enzyme group and no enzyme group, the rates of enzymatic hydrolysis yield using alginate lyase-complex enzyme increased by 53.2%, 174.5% and 245.1%, respectively. Na⁺ and Mg²⁺can raise efficiency on enzymatic hydrolysis yield of kelp. The soluble solids of kelp enzymatic hydrolysate can be increased to 61.3 g/L. In the process of kelp hydrolysis reaction, alginate lyase played a very important role in promoting the degradation effect of kelp. Alginate lyase can increase the content of total sugar, reducing sugar, organic matter and various minerals.