转录组分析毛白杨响应氧化胁迫的基因表达模式变化
Transcriptome Analysis of Gene Expression Patterns of Populus tomentosa in Response to Oxidative Stress
DOI: 10.12677/BR.2018.72025, PDF,  被引量    科研立项经费支持
作者: 李嘉鑫, 李 盈, 滕晓瞳, 李聪慧, 李 慧, 陆 海：北京林业大学生物科学与技术学院，北京
关键词: 毛白杨；氧化胁迫；光合作用；转录组；Populus tomentosa； Oxidative Stress； Photosynthesis； Transcriptome
摘要: 为了了解基因水平上毛白杨对于适应氧化胁迫的分子机制，本研究在氧化胁迫下对毛白杨叶片进行转录组测序分析，将有显著性差异的基因做GO功能及KEGG Pathway的富集分析，分析差异表达基因得出，胁迫与未胁迫毛白杨组之间的总表达基因数为3495，其中上调基因个数为1683，下调基因个数为1812。通过比较胁迫与未胁迫毛白杨组之间的go功能和KEGG分析得出，氧化胁迫后的植株中，与光合作用中光系统I，光系统Ⅱ相关蛋白均显著性下调。与光合膜，类囊体膜结构中基粒的堆积和膜重组相关蛋白均显著性下调(P < 0.05)。说明氧化应激条件下，叶绿体是活性氧的主要产生部位。ROS的大量积累使叶绿体及细胞产生氧化损伤，导致光合作用相关功能下降，光合膜系统受到损伤。
Abstract: In order to understand the molecular mechanism of Populus tomentosa on adaptation to oxidative stress at gene level, the transcriptome analysis of Populus tomentosa leaves under oxidative stress was carried out. GO genes and GO enrichment analysis of KEGG Pathway were analyzed Differen-tially expressed genes showed that there were 3495 total expressed genes between stressed and untamed Populus tomentosa groups, including 1683 up-regulated genes and 1812 down-regulated genes. By comparing the function of GO and KEGG between Populus tomentosa group and untreated Populus tomentosa group, it was found that the photosystem PSI and PSII related proteins in photosynthesis were significantly down-regulated in the plants after oxidative stress. And photo-synthetic membrane, thylakoid membrane structure of the basement membrane accumulation and membrane recombinant protein were significantly downregulated (P < 0.05). Explaining the oxidative stress conditions, chloroplast is the main active oxygen generating site. The massive ac-cumulation of ROS causes oxidative damage to chloroplasts and cells, leading to the decline of photosynthesis-related functions and the damage of the photosynthetic membrane system.
文章引用：李嘉鑫, 李盈, 滕晓瞳, 李聪慧, 李慧, 陆海. 转录组分析毛白杨响应氧化胁迫的基因表达模式变化[J]. 植物学研究, 2018, 7(2): 186-195. https://doi.org/10.12677/BR.2018.72025

参考文献

[1] 陈洁, 林栖凤. 逆境下植物活性氧的代谢[C]//全国农业生物化学与分子生物学学术交流会. 2002.
[2] 薛鑫, 张芊, 吴金霞. 植物体内活性氧的研究及其在植物抗逆方面的应[J]. 生物技术通报, 2013(10): 6-11.
[3] Davletova, S., Rizhsky, L., Liang, H., et al. (2005) Cytosolic Ascorbate Peroxidase 1 Is a Central Component of the Reactiveoxygen Gene Network of Arabidopsis. Plant Cell, 17, 268.
[4] Mittler, R. (2002) Oxidative Stress, Antioxidants and Stress Tolerance. Trends in Plant Science, 7, 405-410.
[5] 冯建灿, 毛训甲, 胡秀丽. 光氧化胁迫条件下叶绿体中活性氧的产生、清除及防御(英文) [J]. 西北植物报, 2005, 25(7): 1487-1498.
[6] 陈花, 吴俊林, 李晓军. 叶绿体中活性氧的产生和清除机制[J]. 现代生物医学进展, 2008, 8(10): 1979-1981.
[7] Li, R., Yu, C., Li, Y., Lam, T.-W., Yiu, S.-M., Kristiansen, K. and Wang, J. (2009) SOAP2: An Improved Ultrafast Tool for Short Read Alignment. Bioinformatics, 25, 1966-1967.
[8] Wang, Z., Gerstein, M. and Snyder, M. (2009) RNA-Seq: A Revolutionary Tool for Transcriptomics. Nature Reviews Genetics, 10, 57-63.
[9] Mortazavi, A., Williams, B.A., McCue, K., Schaeffer, L. and Wold, B. (2008) Mapping and Quantifying Mammalian Transcriptomes by RNA-Seq. Nature Methods, 5, 621-628.
[10] Young, M.D., Wakefield, M.J., Smyth, G.K. and Oshlack, A. (2010) Gene Ontology Analysis for RNA-seq: Accounting for Selection Bias. Genome Biology, 11, R14.
[11] 丁昌俊, 张伟溪, 高暝, 等. 不同生长势美洲黑杨转录组差异分析[J]. 林业科学, 2016, 52(3): 47-58.
[12] Day, R.B., Shibuya, N. and Minami, E. (2003) Identification and Characterization of Two New Members of the GRAS Gene Family in Rice Responsive to N-acetylchitooligosaccharide Elicitor. Biochimica et Biophysica Acta, 1625, 261-268.
[Google Scholar] [CrossRef] 
[13] Gsc, A., Torres, L.F., Déchamp, E., et al. (2017) Differential Fine-Tuning of Gene Expression Regulation in Coffee Leaves by CcDREB1D Promoter Haplotypes under Water Deficit. Journal of Experimental Botany, 68, 3017.
[Google Scholar] [CrossRef] [PubMed]
[14] Fode, B., Siemsen, T., Thurow, C., et al. (2008) The Arabidopsis GRAS Protein SCL14 Interacts with Class II TGA Transcription Factors and Is Essential for the Activation of Stress-Inducible Promoters. Plant Cell, 20, 3122-3135.
[Google Scholar] [CrossRef] [PubMed]
[15] 翟玉山, 邓宇晴, 董萌, 等. 甘蔗捕光叶绿素a/b结合蛋白基因ScLhca3的克隆及表达[J]. 作物学报, 2016, 42(9): 1332-1341.
[16] 许冰清, 安苗苗, 姜可以, 等. 花叶矢竹叶绿体psbD基因的克隆与功能分析[J]. 浙江农林大学学报, 2015, 32(4): 557-565.
[17] Ugalde, J.M., Rodriguez-Furlã, N.C., Rycke, R., et al. (2016) Phosphatidylinositol 4-Phosphate 5-kinases 1 and 2 Are Involved in the Regulation of Vacuole Morphology during Arabidopsis Thaliana Pollen Development. Plant Science, 250, 10-19.
[Google Scholar] [CrossRef] [PubMed]
[18] Pietrzykowska, M., Suorsa, M., Semchonok, D.A., et al. (2014) The Light-Harvesting Chlorophyll a/b Binding Proteins Lhcb1 and Lhcb2 Play Complementary Roles during State Transitions in Arabidopsis. Plant Cell, 26, 3646-3660.
[Google Scholar] [CrossRef] [PubMed]
[19] De, B.S., Betterle, N., Kouril, R., et al. (2011) Arabidopsis Mutants Deleted in the Light-Harvesting Protein LHCB4 have a Disrupted Photosystem II Macrostructure and Are Defective in Photoprotection. Plant Cell, 23, 2659-2679.
[Google Scholar] [CrossRef] [PubMed]
[20] 王罗霞, 张盛敏, 陈银华. 活性氧和一氧化氮在植物-病原体互作反应中的作用[J]. 热带农业科学, 2009, 29(6): 77-84.
[21] Habeck, G., Ebner, F.A., Shimada-Kreft, H., et al. (2015) The Yeast ERAD-C Ubiquitin Ligase Doa10 Recognizes an Intramembrane Degron. Journal of Cell Biology, 209, 261-267.
[Google Scholar] [CrossRef] [PubMed]
[22] 冷静. 高等植物光系统II捕光天线结构与功能的研究[D]: [博士学位论文]. 北京: 中国科学院研究生院中学院大学, 2002.
[23] Ang, H., Liu, J., Wen, X., et al. (2015) Molecular Mechanism of Photosystem I Assembly in Oxygenic Organisms. Biochimica et Biophysica Acta, 1847, 838-848.
[Google Scholar] [CrossRef] [PubMed]
[24] Agdalena, P., Serena, S., Lotta, V.S., et al. (2016) Functional Update of the Auxiliary Proteins PsbW, PsbY, HCF136, PsbN, TerC and ALB3 in Maintenance and As-sembly of PSII. Frontiers in Plant Science, 7, 423.
[25] Uchert, F., Bailleul, B. and Hisabori, T. (2017) A γ-Subunit Point Mutation in Chlamydomonas reinhardtii, Chloroplast F1Fo-ATP Synthase Confers Tolerance to Reactive Oxygen Species. Biochimica et Biophysica Acta—Bioenergetics, 1858, 966-974.
[Google Scholar] [CrossRef] [PubMed]
[26] Bouché, I., Fait, A., Bouchez, D., et al. (2003) Mitochondrial Succinic-Semialdehyde Dehydrogenase of the γ-Aminobutyrate Shunt Is Required to Restrict Levels of Reactive Oxygen Intermediates in Plants. Proceedings of the National Academy of Sciences of the United States of America, 100, 6843-6848.
[Google Scholar] [CrossRef] [PubMed]
[27] Xu, S., Li, J., Zhang, X., et al. (2006) Effects of Heat Acclimation Pretreatment on Changes of Membrane Lipid Peroxidation, Antioxidant Metabolites, and Ultrastructure of Chloroplasts in Two Cool-Season Turfgrass Species under Heat Stress. Environmental and Experimental Botany, 56, 274-285.
[Google Scholar] [CrossRef] 
[28] Bang, W.Y., Jeong, I.S., Kim, D.W., et al. (2008) Role of Arabidopsis CHL27 Protein for Photosynthesis, Chloroplast Development and Gene Expression Profiling. Plant and Cell Physiology, 49, 1350-1363.
[Google Scholar] [CrossRef] [PubMed]
[29] Pietrzykowska, M., Suorsa, M., Semchonok, D.A., et al. (2014) The Light-Harvesting Chlorophyll a/b Binding Proteins Lhcb1 and Lhcb2 Play Complementary Roles during State Transitions in Arabidopsis. Plant Cell, 26, 3646-3660.
[Google Scholar] [CrossRef] [PubMed]
[30] 杨文英, 徐江, 等. 渗透胁迫下水稻幼苗中叶绿素降解的活性氧损伤作用[J]. 植物学报, 1994, 36: 289-295.