拟南芥SSCD1基因启动子与GUS重组载体的构建与转化

Construction and Transformation of Recombinant Plasmid of Gene SSCD1 Promoter and GUS Gene in Arabidopsis thaliana

摘要: 酪氨酸降解途径是动物中一条重要的代谢途径，而在植物中对该途径了解较少。我们前期研究发现：在模式植物拟南芥中，SSCD1基因编码酪氨酸降解途径最后一个酶——延胡索酰乙酰乙酸酶(fumaryl acetoacetate hydrolase)，该基因突变后导致拟南芥在短日照下出现细胞死亡。为了探讨SSCD1基因的组织表达特征，本研究构建了拟南芥SSCD1基因启动子与GUS的融合表达载体并转入拟南芥，得到其转基因植株，经GUS组织化学染色，发现SSCD1基因启动子驱动的GUS基因在下胚轴中表达较强，子叶和根中表达较弱，而在真叶中几乎没有表达。该研究结果表明SSCD1基因在下胚轴中发挥着更重要的作用，对深入探讨植物中酪氨酸降解途径具有重要的作用。

Abstract: The tyrosine degradation pathway is an important metabolic pathway in animals, but in plants, less is known about this pathway. Our previous study found that: in Arabidopsis thaliana, the SSCD1 gene encodes the final enzyme of the tyrosine degradation pathway, fumarate lace to acetate hydrolase (FAH), and mutation of this gene results in cell death in Arabidopsis under short day. To investigate the tissue expression characteristics of the SSCD1 gene, a fusion expression vector of Arabidopsis thaliana> SSCD1 gene promoter and GUS gene was constructed and transformed into Arabidopsis thaliana>, and the transgenic plant was obtained and identified by GUS histochemical staining. The GUS histochemical staining showed that the GUS gene driven by the SSCD1 gene promoter was strongly expressed in the hypocotyls, but weakly in the cotyledons and roots. However, the expression was almost absent in the true leaves. The results of this study indicate that the SSCD1 gene plays a more important role in hypocotyls, which is very important for deeply exploring tyrosine degradation pathway in plants.