基于方酸菁的近红外探针分子通过颜色和比率荧光响应高效检测生物硫醇
A Highly Sensitive Squaraine-Based NIR Probe for Biothiols with Colorimetric and Ratiometric Changes
摘要: 我们设计并合成了一种基于对称结构的方酸衍生物(Sq-TPE)的近红外(NIR)探针用于高效检测生物硫醇。Sq-TPE的吸收和发射峰均在700 nm以上。在测试的多种氨基酸和生物小分子中,Sq-TPE对硫醇具有较高的灵敏度和选择性。三种生物硫醇的加入使Sq-TPE的颜色发生了从青色到黄色的显著变化,同时,荧光发射从红光变为橙光。此外,Sq-TPE对Cys,GSH和HCy的检测限分别为9.8 nM, 12.4 nM,和9.7 nM。
Abstract: In order to detect high sensitivity and selective biothiol, we designed and synthesized a NIR probe based on a symmetrical squaraine derivative (Sq-TPE), with absorption and emission bands beyond 700 nm. Sq-TPE exhibits high sensitivity and selectivity towards biothiols over the other amino acids and bioanalytes. The addition of three biothiols results in significant color changes of Sq-TPE from cyan to yellow, and meanwhile ratiometric emission changes from red to orange. More excitingly, it shows detection limits as low as 9.8 nM for Cys, 12.4 nM for GSH and 9.7 nM for HCy, respectively.
文章引用:李施汶, 乔建珲, 罗丹, 朱琳娜. 基于方酸菁的近红外探针分子通过颜色和比率荧光响应高效检测生物硫醇[J]. 材料科学, 2018, 8(5): 548-558. https://doi.org/10.12677/MS.2018.85064

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