功能化纳米金检测铜金属离子

Investigation of Functionalized Au Nanoparticles for Copper Ions

摘要: 采用柠檬酸钠还原法制备纳米金颗粒(AuNPs)作为比色溶液，将木瓜蛋白酶修饰于AuNPs 表面，形成稳定的木瓜蛋白酶- AuNPs水溶复合物，根据木瓜蛋白酶和Cu²⁺之间的特异性结合引起木瓜蛋白酶- AuNPs聚集，使溶液颜色由红色变为蓝紫色，实现了水中Cu²⁺的比色检测。实验结果显示该方法的检测范围为0.5~25 μg∙mL⁻¹，检出限为0.3 μg∙mL⁻¹，其他重金属离子干扰较小。该方法具有制备和操作简单等优点，可望用于水体中Cu²⁺的现场快速检测。

Abstract: Sodium citrate reduction method was used to prepare nano gold particles (AuNPs) as a colorimetric solution. Papain was modified on the surface of AuNPs to form a stable papain AuNPs water soluble complex. According to the specific binding between papain and Cu²⁺, papain-AuNPs aggregation was induced, and the color of the solution changed from red to blue violet. The colorimetric detection of Cu²⁺ in water is realized. The experimental results show that the detection range of the method is 0.5~25 μg∙mL⁻¹, the detection limit is 0.3 μg∙mL⁻¹, and other heavy metal ions have little interference. The method is simple and easy to prepare. It can be used for rapid detection of Cu²⁺ in water.