高效液相色谱法检测食品接触材料甲醛和2-甲酚聚合物中甲醛的迁移量

Determination of Formaldehyde in Formaldehyde and 2-Cresol Polymers by Foodstuffs by HPLC

摘要: 采用高效液相色谱法测定甲醛和2-甲酚聚合物中的甲醛在食品模拟物中的迁移规律。样品以食品模拟物进行浸泡提取后，加入2,4-二硝基苯肼，与浸泡液中的甲醛反应生成2,4-二硝基苯腙，以C18色谱柱为分离柱，以甲醇-水(6 + 4)溶液为流动相进行等度洗脱，检测波长为355 nm。甲醛的质量浓度在0.01~40.0 mg/kg范围内呈线性。对空白样品进行加标回收试验，回收率在98.5%~101.2%之间，相对标准偏差(n = 10)均不大于1.1%。该方法适用于甲醛和2-甲酚聚合物中甲醛迁移量的检测，检测结果显示，在相同的使用条件下，酸性模拟物中甲醛的迁移量最大；在接触同种食品模拟物时，甲醛的迁移量随着接触时间的延长和温度的升高而增加。

Abstract: The migration of formaldehyde in food analogues was determined by high performance liquid chromatography (HPLC). After the sample was extracted with the food simulant, 2,4-dinitrophenylhydrazone was added and reacted with formaldehyde in the soaking solution to form 2,4-dinitrophenylhydrazone. The C18 column was used as the separation column. The water (6 + 4) solution was eluted with the mobile phase, and the detection wavelength was 355 nm. The mass concentration of formaldehyde is linear in the range of 0.01 - 40.0 mg/kg. The recoveries were between 98.5% and 101.2%, and the relative standard deviations (n = 10) were not more than 1.1%. The method is applied to the detection of formaldehyde transport in formaldehyde and 2-cresol polymers. The results show that the migration of formaldehyde in acidic mimics is the largest under the same conditions of use. When contacting the same food simulator, the amount of migration increases as the contact time increases and the temperature increases.