基于阿霉素–嵌段共聚物的多功能纳米载药体系及其性能研究

Study of Multifunctional Nano-Drug Loading System and Its Properties Based on the Doxorubicin-Block Copolymer

摘要: 目的：以阿霉素(DOX)为模型药物，嵌段共聚物(PMMA-co-MAA)为纳米载体，构建一种新型的多功能纳米载药体系，并对其性能进行研究。方法：基于PMMA-co-MAA在水溶液中自组装形成“外亲水，内疏水”纳米胶束的原理，采用共沉淀–自组装法来制备DOX@PMMA-co-MAA纳米载药体系。通过TEM、DLS和zeta电位等进行形貌和表面电荷的表征。同时，采用紫外和荧光光谱法对其性能进行考察。结果：纳米颗粒平均粒径约为50 nm，表面电荷为−36.9 mV。在470 nm处具有较强的紫外吸收，最大发射波长为563 nm。同时，在缓冲液和血清中具有优良的荧光特性和光学稳定性。结论：本实验通过共聚物PMMA-co-MAA对疏水性药物DOX的包裹，明显地改善了药物的光学性能和生物利用度，减少其毒副作用，从而实现了细胞水平上的荧光成像研究。

Abstract: Purpose: A novel multifunctional nano-drug loading system based on the doxorubicin-block copo-lymer was developed and its properties were also studied. Methods: Based on the principle of self-assembly of PMMA-co-MAA in aqueous solution to form “hydrophilic and hydrophobic” na-no-micelles. After all, the morphology and surface charge of nano-drug loading system were inves-tigated by TEM, DLS and zeta potential. At the same time, the properties were also investigated by UV and fluorescence spectroscopy. Results: The average diameter and surface charge of nanopar-ticles were about 50 nm and −36.9 mV, respectively. The maximum absorption wavelength is 478 nm and the maximum fluorescence emission wavelength is 563 nm. It has excellent optical prop-erties and good optical stability in buffer and serum. Conclusion: In this experiment, the optical properties and biocompatibility of drug were improved obviously by means of the encapsulation of hydrophobic DOX by copolymers, reducing side effects and it is expected to be used in the fields of fluorescence imaging at the cell level.