辐照桦褐孔菌提取物抗氧化作用的研究

Study on Antioxidation of Extracts from Irradiated Inonotus obliquus

摘要: 为了研究辐照对桦褐孔菌的抗氧化作用的影响，使用不同辐照剂量，即0 kGy、5 kGy、10 kGy、20 kGy和30 kGy的⁶⁰Co-γ射线进行辐照处理，通过测定辐照桦褐孔菌提取物的总抗氧化能力、DPPH∙清除能力、∙OH清除能力和总还原力来研究辐照桦褐孔菌提取物的抗氧化作用。结果表明，不同辐照剂量的桦褐孔菌的总抗氧化能力之间存在一定的差异，20 kGy辐照处理的桦褐孔菌提取物的总抗氧化能力最高，但是，它显著低于维生素C的总抗氧化能力；在相同浓度下，不同辐照剂量的桦褐孔菌提取物的DPPH∙和∙OH清除能力存在显著差异；对于相同辐照剂量的样品，DPPH∙清除能力和∙OH清除能力随着样品的增加而显著增加，但均显著低于维生素C清除能力(p < 0.05)；不同辐照剂量的桦褐孔菌提取物的总还原力存在波动，但均显著低于维生素C的总还原力。

Abstract: In order to determine the antioxidant effects of irradiation on Inonotus obliquus, 0 kGy, 5 kGy, 10 kGy, 20 kGy and 30 kGy ⁶⁰Co-gamma ray irradiation was applied to Inonotus obliquus. By determinating Inonotus obliquus’s total antioxidant capacity, DPPH∙ scavenging activity, scavenging ∙OH ability and total reduction capacity, the antioxidant effect of radiated Inonotus obliquus extracts was studied. The results show that the total antioxidant capacity was different in different irradiation doses. The total antioxidant capacity of 20 kGy irradiated Inonotus obliquus extract was the highest, but significantly lower than that of vitamin C. At the same concentration of Inonotus obliquus, total antioxidant capacity, DPPH∙ scavenging activity, and scavenging ∙OH ability, total reduction capacity with different irradiation doses were significantly different. With the increase of sample concentration, DPPH∙ scavenging activity, and scavenging ∙OH ability were significantly increased with the increase of sample concentration, but they were all significantly lower than the vitamin C scavenging capacity; the total reduction force of different irradiated doses of Inonotus obliquus was fluctuated, but significantly lower than the total reducing power of vitamin C.