四种补肾中草药中14种元素的测定及统计分析

Determination and Statistical Analysis of 14 Kinds of Elements in Four Kinds of Kidney Tonic Chinese Herbal Medicine

摘要: 本文采用因子分析法、方差分析法及相关分析法分析枸杞、黄精、巴戟天、肉苁蓉四种中药材中14种元素差异的显著性和相关性，为研究中药材微量元素与药效之间的关系提供一定的依据。方法：采用水煮法和微波直接消解法处理样品，以电感耦合等离子发射光谱法(ICP-AES)测定了枸杞、黄精、巴戟天、肉苁蓉中14种元素含量。采用因子分析法、方差分析法及相关分析法对测定结果作统计分析，分析不同补肾中药微量元素差异的显著性和相关性。实验结果表明，4种中药中K，Ca和Na的含量均较高，微波消解法提取的微量元素含量明显高于水煮法，此方法测定各元素的相对标准偏差(RSD)在0.15%~3.65%之间，加标回收率在94%~105.5%之间，表明本文测量结果可准确评估四种中药材中14种元素的含量。不同的提取方法对14种元素有显著影响，四种中药材元素具有相似性，含量差异明显。未来的研究中通过更多种类补肾中药中各种元素的分析，将有助于开拓不同元素在中药材中的功能新领域。

Abstract: We use the method of factor analysis, variance analysis and correlation analysis to analyze the significance and correlation of difference between the 14 elements in four kinds of Chinese medi-cinal materials (Wolfberry, Polygonatum, Morinda officinalis, Cistanche deserticola). This study will provide a basis for the study of the relationship between trace elements and pharmacodynamics in Chinese medicinal herbs. The sample was prepared with water boiling method and microwave digestion, 14 kinds of trace elements in these (Wolfberry, Polygonatum, Morinda officinalis, Cistanche deserticola) herbal medicine was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The experimental results showed that the trace elements of mi-crowave digestion method were significantly higher than that of water boiling method, and the con-tent of K, Ca and Na was very high in four kinds of Chinese herbal medicine. The relative standard deviation (RSD) of each element was between 0.15% and 3.65%, and the recovery rate was between 94% and 105.5%. The results showed that the content of 14 trace elements in four kinds of Chinese medicinal herbs could be accurately evaluated. Different extraction methods have significant effects on 14 kinds of trace elements. The trace elements in four kinds of Chinese medicinal herbs have similar characteristics and the content is obviously different. In the future research, the analysis of trace and macro elements in Chinese herbal medicine of tonifying kidney will help to develop new functional fields of trace and macro elements in Chinese medicinal herbs.