几种茶用菊花在高原环境下引种后主要活性成分含量测定及分析

Determination and Analysis of Main Active Components in Several Tea Chrysanthemum Cultivars after Being Introduced in Plateau Environment

摘要: 目的：利用HPLC测定茶用菊花品种高原环境下引种后提取物中绿原酸、木犀草苷、槲皮素的含量，测定菊花总黄酮含量。方法：采用Aglient SB C18色谱柱(250 mm × 4.6 mm, 5 µm)，以乙腈A-0.5%甲酸水溶液B为流动相，柱温为25℃，流速为0.8 mL/min，进样量为5 µL，检测波长为348 nm。结果：高原环境下引种后，茶用菊花主要活性成分含量均有显著增加(p < 0.05)，不同茶用菊花品系主要活性成分含量存在差异。结论:同一菊花品种不同产区主要活性成分含量存在差异，不同品种菊花主要活性成分含量各异。

Abstract: Objective: HPLC was used to determine the contents of chlorogenicacid, luteoloside, quercetin and total flavonoids in tea chrysanthemum flower extract under plateau area after the introduction. Methods: Using Aglient SB C18 column (250 mm × 4.6 mm, 5 µm), taking acetonitrile A-0.5% aqueous formic acid B as the mobile phase, the column temperature is 25 degrees Celsius, the flow rate is 0.8 mL/min, sample volume is 5 µL, and the detection wavelength is 348 nm. Results: Under the plateau area, the main active components of tea chrysanthemum flower increased significantly after introduction, and the contents of main active components in different tea chrysanthemum flower lines are different (p < 0.05). Conclusion: The main active ingredients in one variety in different areas of chrysanthemum flower are different, and the main active ingredients in different varieties of chrysanthemum flower are different.