柳枝稷“京稷31”八倍体诱导及其表型与生理分析
Induction of Octoploid and Its Phenotypic and Physiological Analysis in Switchgrass “Jingji31”
DOI: 10.12677/WJF.2019.81002, PDF,  被引量    科研立项经费支持
作者: 吴海燕：内蒙古师范大学生命科学与技术学院，内蒙古 呼和浩特；北京市农林科学院草业与环境研究发展中心，北京；范希峰, 武菊英, 腾文军, 张辉, 温海峰, 岳跃森*：北京市农林科学院草业与环境研究发展中心，北京；斯琴巴特尔：内蒙古师范大学生命科学与技术学院，内蒙古 呼和浩特
关键词: 柳枝稷；观赏植物；秋水仙素；八倍体；Switchgrass； Ornamental Plant； Colchicine； Octoploid
摘要: 本研究以观赏植物四倍体高地型柳枝稷“京稷31”的幼穗为外植体诱导出愈伤组织，采用秋水仙素诱导处理获得其同源多倍体。流式细胞仪测定表明，其叶片DNA相对含量为四倍体的2倍；与四倍体相比，八倍体植株叶片下表皮的气孔密度显著降低而气孔大小明显增大(P < 0.01)。表型分析结果表明，八倍体柳枝稷株高、分蘖显著降低(P < 0.01)，而茎粗和叶片宽度显著增加(P < 0.01)，叶长无显著差异；进一步研究表明，八倍体植株的叶绿素含量和光合作用比四倍体植株显著增加(P < 0.05)。
Abstract: In this study, callus was induced from young inflorescences of tetraploid upland switchgrass “Jingji31”, an ornamental plant, and homologous octoploids were obtained by colchicine induction. Detection of Flow cytometry showed that the relative DNA content of leaves in it was twice as high as that of tetraploid. Stomatal frequency reduced but stomatal size increased significantly (P < 0.01) in leaf epidermis of octoploid compared with its tetraploid. Phenotypic analysis showed that plant height and tiller decreased significantly (P < 0.01) in octoploid plants, while stem diameter and leaf width increased significantly (P < 0.01), and leaf length did not change. Moreover, octoploid plants have more chlorophyll content and higher photosynthesis (P < 0.05) than tetraploid.
文章引用：吴海燕, 范希峰, 武菊英, 斯琴巴特尔, 腾文军, 张辉, 温海峰, 岳跃森. 柳枝稷“京稷31”八倍体诱导及其表型与生理分析[J]. 林业世界, 2019, 8(1): 10-16. https://doi.org/10.12677/WJF.2019.81002

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