金枪鱼骨胶原蛋白肽螯合钙对去卵巢大鼠骨质疏松症的影响

Effects of Collagen Peptide from Tuna Bone Chelated Calcium on Osteoporosis in Ovariectomized Rats

摘要: 胶原多肽与骨钙的流失直接导致了骨质疏松症的发生，补充胶原多肽与钙质是预防骨质疏松的有效途径。本文以前期制备的金枪鱼骨胶原蛋白肽(collagen peptide from tuna bone, TCP)螯合钙为受试物，通过双侧摘除卵巢构建的骨质疏松症模型，研究螯合钙抑制骨质疏松症的作用。实验结果显示，螯合钙显著降低了骨质疏松症大鼠血清骨吸收关键因子抗酒石酸酸性磷酸酶、组织蛋白酶K和核因子κB受体活化因子的浓度，抑制骨吸收；螯合钙显著降低了大鼠血清骨形成关键因子骨源性碱性磷酸酶、骨钙素、I型前胶原羧基端前肽的浓度，减小了核因子κB受体活化因子配体和骨保护素的比例，调节骨形成；螯合钙显著增加了股骨密度，增强了胫骨最大载荷和结构强度。提示，金枪鱼骨胶原蛋白肽螯合钙能有效地抑制摘除卵巢大鼠的骨质疏松症，为金枪鱼骨的高值化利用提供了理论依据。

Abstract: The loss of collagen peptide and bone calcium directly induces osteoporosis. Supplementation of collagen peptide and calcium is an effective approach to prevent osteoporosis. In this study, the effects of collagen peptide from tuna bone chelated calcium (TCP/Ca), prepared in the previous experiments, on inhibition of osteoporosis, were investigated in bilateral oophorectomy rats. Results showed that TCP/Ca significantly decreased the concentration of tartaric acid phosphatase, cathepsin K and receptor activator of nuclear factor κB in the serum of osteoporosis rats, and inhibited bone resorption. TCP/Ca significantly reduced serum bone alkaline phosphatase, osteocalcin, propeptide carboxy-terminal procollagen, and also lowered the ratio of receptor activator of nuclear factor κB ligand and osteoprotegerin. TCP/Ca remarkably increased bone mineral density of femur, enhanced maximum load and structural strength of tibia. These indicated that TCP/Ca can effectively inhibit osteoporosis in oophorectomy rats, which provides a theoretical basis for high value utilization of tuna bone.