普鲁兰酶辅助双酶法降解芭蕉芋淀粉的研究
Study on Degradation of Canna Edulis Starch by the Pullulanase-Assisted Double Enzyme Method
DOI: 10.12677/HJFNS.2019.84037, PDF,    科研立项经费支持
作者: 王昌梅, 张无敌*, 尹 芳, 赵兴玲, 杨 斌, 吴 凯, 柳 静, 杨 红, 刘士清：云南师范大学太阳能研究所，云南 昆明；韩 伟, 黄文荣*, 雷 宇, 李万仙：云南师范大学太阳能研究所，云南 昆明；云南万芳生物技术有限公司，云南 昆明
关键词: 普鲁兰酶；双酶法；芭蕉芋淀粉；降解；Pullulanase； Double Enzymatic Method； Canna edulis Ker Starch； Degradation
摘要: 以芭蕉芋淀粉为原料，料水比为1:3进行发酵，确定了普鲁兰酶辅助双酶法对芭蕉芋淀粉的降解情况。通过实验确定普鲁兰酶添加的最适阶段是在液化前添加，与对照组相比，在液化前先添加普鲁兰酶30 µ/g，可使还原糖的含量提高1.29%，发酵结束后的酒精质量分数提高1.3，结果表明添加普鲁兰酶有助于双酶法降解芭蕉芋淀粉。
Abstract: Using Canna edulis Ker starch as raw material, the ratio of material to water was 1:3, and the de-gradation of Canna edulis Ker starch was studied by the pullulanase-assisted double enzyme me-thod. It was determined by experiments that the optimum stage of the addition of pullulanase was added before liquefaction. Compared with the control group, the addition of pullulanase 30 µ/g before liquefaction can increase the content of reducing sugar by 1.29%, and the alcohol content after fermentation was increased by 1.3. The results indicated that the addition of pullulanase was helpful for the double enzymatic degradation of Canna edulis Ker starch.
文章引用：王昌梅, 张无敌, 韩伟, 黄文荣, 雷宇, 李万仙, 尹芳, 赵兴玲, 杨斌, 吴凯, 柳静, 杨红, 刘士清. 普鲁兰酶辅助双酶法降解芭蕉芋淀粉的研究[J]. 食品与营养科学, 2019, 8(4): 285-290. https://doi.org/10.12677/HJFNS.2019.84037

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