大田基黄总黄酮含量测定及提取物抗乙肝病毒作用
Determination of Flavonoids and Anti-Heptitis B Virus Activity of Extracts from Lysimachia fortunei Maxim
DOI: 10.12677/HJFNS.2020.91012, PDF,    科研立项经费支持
作者: 龚受基：北部湾大学食品工程学院，广东 钦州；桂林医学院，广西 桂林；杨 茵：桂林医学院，广西 桂林
关键词: 大田基黄；黄酮；HepG2.2.15细胞系；Lysimachia fortunei Maxim； Flavonoid； HepG2.2.15 Cells
摘要: 目的：测定大田基黄提取物中总黄酮含量，研究大田基黄不同提取物体外抗乙肝病毒作用。方法：采用大孔吸附树脂(D101)纯化工艺技术，对大田基黄提取物进行提纯，应用NaNO2-AlCl3显色法测定总黄酮含量；应用HepG2.2.15细胞系培养系统检测大田基黄提取物对HBsAg和HBeAg的抑制作用。结果：经树脂纯化后的大田基黄提取物总黄酮含量达89.29%；大田基黄提取物对HBsAg表达有抑制作用，其中对HBsAg抑制作用差别较大。结论：大孔吸附树脂(D101)分离纯化大田基黄总黄酮效果显著；大田基黄提取物有体外抗乙肝病毒作用。
Abstract: Objective: To determinate the flavonoids’ content and the anti-hepatitis B virus (HBV) effect of the extracts from Lysimachia fortunei Maxim in vitro. Methods: Macroporous resin (D101) was used to isolate and purify the extracts of Lysimachia fortunei Maxim and the total flavonoids’ content was determinated by NaNO2-AlCl3 system. Then, the HepG2.2.15 cell line was used to investigate the anti-HBV effects of the extract from Lysimachia fortunei Maxim. Results: The content of flavonoids reaches 89.29%. The extracts are shown to inhibit the HBeAg secretion in HepG2.2.15 cells. Conclusions: Macroporous resin (D101) is efficient for purification of total flavonoids from Lysimachia fortunei Maxim. The extracts of Lysimachia fortunei Maxim are effective in inhibition of HBV in vitro.
文章引用：龚受基, 杨茵. 大田基黄总黄酮含量测定及提取物抗乙肝病毒作用[J]. 食品与营养科学, 2020, 9(1): 95-100. https://doi.org/10.12677/HJFNS.2020.91012

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