六堡茶茶褐素对小鼠肠道菌群结构的影响

The Influence of Liupao Tea Theabrownins on the Profile of Gut Microbiota in Mice

摘要: 目的：探讨六堡茶茶褐素对肠道菌群结构的影响。方法：以普通饲料和高脂饲料喂养C57BL/6J小鼠进行造模。造模成功后，将小鼠分为普通对照组(N组)、普通肥胖组(NT组)、高脂对照组(HFD组)和高脂肥胖组(HFDT组)，四组小鼠分别给予普通饲料、普通饲料加药物、高脂饲料、高脂饲料加药物喂养。分别在给药前和给药八个月后采集4组小鼠粪便样本并提取菌群基因，分析其肠道物种菌群组成。结果：造模结束后，肥胖小鼠肠道中拟杆菌丰度、拟杆菌/厚壁菌比值降低，给药增加了拟杆菌丰度、拟杆菌/厚壁菌比值，降低了厚壁菌丰度。结论：六堡茶茶褐素对小鼠肠道拟杆菌、厚壁菌结构和数量有显著影响，可以改善小鼠肠道菌群结构。

Abstract: Objective: To investigate the effect of Liupao tea theabrownins on intestinal microflora. Methods: C57BL/6J mice were fed with normal diet and high fat diet. After the model was established successfully, the mice were divided into four groups: normal diet group (N Group), normal diet + obesity group (NT group), high fat diet group (HFD group) and high fat diet + obesity group (HFDT group). The four groups were fed with normal diet, normal diet plus Liupao tea theabrownins, high fat diet, high fat feed plus Liupao tea theabrownins respectively. Fecal samples of 4 groups of mice were collected before and after eight months of administration, and bacterial genes were extracted to analyze the composition of intestinal flora by 16S rDNA analysis. Results: At the end of modeling, the abundance of Bacteroides and the ratio of Bacteroides to sclerella decreased in the intestine of obese mice. After administration, Bacteroides abundance and Bacteroides/sclerella ratio were increased, while sclerella abundance was decreased. Conclusion: The structure and quantity of Bacteroides and sclerella in intestine of mice were significantly affected by theabrownins from Liupao tea, which can ameliorate the structure of intestinal flora.