茶树油纳米乳液抑菌活性以及促进皮肤创伤愈合作用研究

Study on the Antibacterial Activity and Promoting Skin Wound Healing Activities of Tea Tree Oil Nanoemulsion

摘要: 茶树油是从互叶白千层茶树提取获得的精油，具有显著的抑菌活性。本研究以茶树油作为油相，吐温20作为乳化剂，大豆磷脂作为助乳化剂，蒸馏水作为水相，利用超声波处理制备o/w型纳米乳液，壳聚糖包埋处理乳液制备壳聚糖包埋纳米乳液。经过条件优化后，纳米乳液平均直径为36nm左右，PDI小于0.3，表明纳米乳液分布范围集中，稳定性较高。不经壳聚糖包埋的纳米乳液zeta值为−20mV左右，经过壳聚糖包埋处理后，zeta值变为15 mV左右，说明壳聚糖包埋成功。抑菌圈检测纳米乳液的抑菌活性，说明纳米乳液可以显著抑制大肠杆菌和金黄色葡萄球菌生长。茶树油纳米乳液在一定程度上可以促进皮肤创伤组织愈合，表明茶树油纳米乳液具有皮肤保护作用。

Abstract: Tea tree oil (TTO) is an essential oil extracted from Melaleuca alternifolia tea tree leave and has significant antibacterial activity. In this study, tea tree oil was used as the oil phase, Tween 20 was used as the emulsifier, soybean phospholipids were used as co-emulsifiers, and distilled water was used as the water phase. O/W nanoemulsions were prepared by ultrasonic treatment, and chitosan was embedded to coat the TTO nanoemulsion. After the optimization of the conditions, the average diameter of the nanoemulsion is about 36 nm, and the PDI is less than 0.3, which indicates that the nanoemulsion has a concentrated distribution range and high stability. The zeta value of the nanoemulsion without chitosan coating was about −20 mV. After the chitosan coating treatment, the zeta value became about 15 mV, indicating that the chitosan was successfully coated. The antibacterial activity of the nanoemulsion was tested by the zone of inhibition, indicating that the nanoemulsion can significantly inhibit the growth of E. coli and S. aureus. Tea tree oil nanoemulsion can promote skin wound tissue healing to a certain extent, indicating that tea tree oil nanoemulsion has skin protection ability.