睡眠剥夺对抑郁模型大鼠海马盐皮质激素受体的影响及其机制的探讨
Effects and Mechanisms of Sleep Deprivation on Hippocampal Mineralocorticoid Receptor (MR) in Depressed Rats
DOI: 10.12677/TCM.2020.92031, PDF,    科研立项经费支持
作者: 李 星, 王秀峰, 张 瑜, 曾雪爱, 张一帆：福建省中医药科学院，福建省中医睡眠医学重点实验室，福建 福州
关键词: 睡眠剥夺；抑郁；盐皮质激素受体；Sleep Deprivation； Depression； Mineralocorticoid Receptor
摘要: 目的：观察睡眠剥夺防治抑郁症的作用与盐皮质激素受体(Mineralocorticoid Receptor, MR)的相关性。方法：利用慢性应激抑郁大鼠模型，造模成功后分别分为对照组、模型组、西药组以及睡眠剥夺组。每周测量大鼠的体重变化。睡眠剥夺采用改良多平台睡眠剥夺法(modified multiple platform method, MMPM)。通过Morris水迷宫实验对大鼠空间学习记忆能力进行测试。运用Western Blotting实验检测大脑海马组织MR蛋白表达水平。结果：同对照组相比较，模型组大鼠体重量显著下降(P < 0.01)，大鼠的逃避潜伏期显著延长(P < 0.01)、跨越平台次数显著减少(P < 0.01)，海马组织中MR蛋白表达水平被显著下调(P < 0.01)。同模型组相比，睡眠剥夺组大鼠体重量显著增加(P < 0.01)，大鼠的逃避潜伏期显著缩短(P < 0.05)、跨越平台次数显著增加(P < 0.05)，海马组织中MR蛋白表达水平被显著上调(P < 0.01)。结论：睡眠剥夺可以改善慢性应激抑郁模型大鼠的生长状态，提升模型大鼠空间学习和记忆能力，并且其作用机制可能与大鼠海马盐皮质激素受体途径有关。
Abstract: Objective: To observe the effects of sleep deprivation on depression and the correlation of Mine-ralocorticoid Receptor (MR). Methods: The rats with chronic stress depression were divided into control group, model group, western medicine group and sleep deprivation group. Weight changes were measured weekly. The modified multiple platform method (MMPM) was used for sleep de-privation. The abilities of spatial learning and memory were tested by Morris water maze experi-ment. Western Blotting was used to detect the expression level of MR protein in hippocampus. Results: Compared with the control group, the weight of the rats in the model group decreased significantly (P < 0.01), the evading incubation period of the rats was significantly prolonged (P < 0.01), and the number of crossing the platform was significantly decreased (P < 0.01). The expres-sion level of MR protein in the hippocampus was significantly decreased (P < 0.01). Compared with the model group, the weight of the rats in the sleep deprivation group increased significantly (P < 0.01), the escape latency period of the rats was significantly shortened (P < 0.05), and the number of platform crossings was significantly increased (P < 0.05). The expression level of MR protein in the hippocampus was significantly increased (P < 0.01). Conclusion: Sleep deprivation can improve the growth state of the rats with chronic stress and depression, improve the spatial learning and memory ability of the rats, and its mechanism may be related to the hippocampal mineralocorticoid receptor pathway.
文章引用：李星, 王秀峰, 张瑜, 曾雪爱, 张一帆. 睡眠剥夺对抑郁模型大鼠海马盐皮质激素受体的影响及其机制的探讨[J]. 中医学, 2020, 9(2): 217-224. https://doi.org/10.12677/TCM.2020.92031

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