低表达miR-210-3p调控大鼠垂体瘤GH3与MMQ细胞株增殖与迁移

The Low Expression of miR-210-3p Regulates the Proliferation and Migration of GH3 and MMQ Cell Lines in Rat Pituitary Adenoma

摘要: 目的：探讨低表达miR-210-3p对大鼠垂体瘤GH3和MMQ细胞株的增殖与迁移的作用。方法：将培养好的大鼠垂体瘤MMQ与GH3细胞株随机分为低表达miR-210-3p组和阴性对照组，分别转染miR-210-3p inhibitor和miR-210-3p NC，荧光定量PCR检测实验组与对照组的miR-210-3p的表达；转染后24 h、48 h、72 h分别进行CCK8检测增殖能力；用Transwell来检测大鼠垂体瘤细胞的迁移能力；用Western- boltting技术分别检测低表达miR-210-3p组和阴性对照组的FGFRL-1的蛋白表达；用双荧光素酶实验验证miR-210-3p的靶基因。结果：转染低表达病毒后MMQ与GH3垂体瘤细胞株的细胞增殖能力下降较阴性对照组比较差异，P < 0.05，有统计学意义；低表达miR-210-3p组MMQ与GH3垂体瘤细胞系与阴性对照组相比细胞迁移能力下降，P < 0.05，有统计学意义；转染低表达病毒组的细胞株与对照组相比，靶基因蛋白表达有差异，P < 0.05；miR-210-3p可与FGFRL-1 mRNA的3’-UTR结合，FGFRL-1为miR-210-3p的靶基因。结论：miR-210-3p低表达抑制大鼠垂体瘤细胞的增殖与迁移。

Abstract: Objective: To investigate the role of miR-210-3p in regulating the proliferation and migration of GH3 and MMQ cell lines. Methods: The cultured rat pituitary adenoma MMQ and GH3 cell lines were randomly divided into the low-expression miR-210-3p group and the negative control group. The expression of miR-210-3p was transfected with miR-210-3p inhibitor and miR-210-3p NC, respectively. After transfection, proliferation capacity of CCK8 was detected at 24 h, 48 h and 72 h, respectively. Transwell was used to detect the migration ability of pituitary tumor cells in rats. The protein expression of fgfrl-1 in the low miR-210-3p group and the negative control group was detected by western-boltting technique. The target genes of miR-210-3p were verified by double luciferase assay. Results: After transfection with the low expression virus, the decreased cell proliferation of the pituitary adenoma cell lines MMQ and GH3 was significantly lower than that of the negative control group (P < 0.05). Compared with the negative control group, MMQ and GH3 pituitary adenoma cell lines in the low-expression miR-210-3p group showed decreased cell migration, P < 0.05, which was statistically significant. Compared with the control group, the expression of target gene protein was different in the transfected low-expression virus group (P < 0.05). miR-210-3p can bind to 3'-utr of FGFRL-1 mRNA, and FGFRL-1 is the target gene of miR-210-3p. Conclusion: The low expression of miR-210-3p inhibited the proliferation and migration of MMQ and cells.