大鼠创伤性脑损伤中差异表达基因microRNA调节网络的生物信息学研究

Bioinformatics Study of Differentially Expressed Genes microRNA Regulatory Network in Traumatic Brain Injury in Rats

摘要: 探究大鼠创伤性脑损伤(traumatic brain injury, TBI)基因表达差异同miRNA的调节网络，为阐明TBI治疗靶点提供理论基础，并以期寻找TBI临床诊断的客观指标。本研究实验方法使用美国国立生物技术信息中心(NCBI)，从GEO数据库获得有关TBI基因表达与miRNA表达的原始数据。通过筛选差异表达基因和miRNA，并经过一系列生物信息学软件处理，对差异表达的miRNA进行靶基因预测，将系统提供的靶基因与差异表达基因进行比较，保留重合的差异表达基因构建了miRNA-Gene靶向调控网络，并对差异表达的基因进行了基因本体论(GO)功能富集分析。本研究结果最终筛选出95个差异表达基因和4个靶向miRNA。利用筛选结果构建完成了miRNA-Gene靶向调控网络，并通过GO功能富集分析，得到了从生物学过程、细胞成分及分子功能三个角度对这些基因进行的功能描述。miRNA-Gene靶向调控网络展示了创伤性脑损伤后相关miRNA与其靶基因是如何通过相互作用而进一步产生一系列生物学效应的。本研究通过GO功能富集分析和调控网络，有助于理解和阐明TBI的潜在机制和miRNA调节的关系，并为TBI临床诊断提供了4个可能选择的生物标志物。

Abstract: To explore the difference of gene expression and the regulatory network of miRNA in rats with traumatic brain injury (TBI), so as to provide a theoretical basis for elucidating the therapeutic targets of TBI, and to find the objective indicators for clinical diagnosis of TBI, in this study, the Na-tional Center for Biotechnology Information (NCBI) was used to obtain the original data on TBI gene expression and miRNA expression from GEO database. Through the screening of differentially ex-pressed genes and the miRNA and a series of bioinformatics software processing, we performed target gene prediction for differentially expressed of miRNAs. By comparing the target genes pro-vided by the system with the differentially expressed genes, the miRNA-gene targeting regulatory network was constructed by using the differentially expressed genes that were repeated twice, and the Gene Ontology (GO) functional enrichment analysis of differentially expressed genes was con-ducted. There are 95 differentially expressed genes and 4 targeted miRNAs in the study were se-lected. The miRNA-gene targeting regulatory network was constructed based on the screening re-sults, and the functional description of these genes was obtained from the biological process, cellu-lar components and molecular functions through GO functional enrichment analysis. The miR-NA-gene targeting regulatory network demonstrates how related miRNAs and their target genes interact to further produce a series of biological effects after TBI. Through GO functional enrichment analysis and regulatory network, this study helps to understand and clarify the potential mecha-nism of TBI and the relationship between miRNA regulation, and provides four possible biomarkers for the clinical diagnosis of TBI.