强直性脊柱炎患者口腔菌群的多样性及差异性研究

Diversity and Difference of Oral Flora in Patients with Ankylosing Spondylitis

摘要: 目的：通过高通量测序探究强直性脊柱炎患者口腔菌群的紊乱情况，分析口腔菌群与疾病活动度的相关性。方法：选取强直性脊柱炎患者66名，健康对照者31名纳入本次研究。收集所有研究对象的非刺激性唾液，提取DNA进行16SrRNA高通量测序，检测所有研究对象的口腔菌群组成，并进行菌群多样性分析，分析AS患者与健康对照者的菌群差异。收集患者临床信息，分析菌群与临床疾病活动的相关性。结果：我们的研究在所有受试者口腔中共发现菌群17个门(Phylum)，32个纲(Class)，63个目(Order)，115个科(Family)，224个属(Genus)。ɑ多样性分析显示强直性脊柱炎患者的口腔菌群丰度及多样性大于健康组(P < 0.05)。β多样性分析显示患者口腔菌群的组成和健康组之间存在一定差异，但无法完全分开(P > 0.05)。差异性分析显示患者口腔菌群在门水平有1种菌群，在科水平有3种菌群，在属水平有11种菌群的丰度与健康志愿者有差异(P < 0.05)。相关性分析显示在属水平有29种菌群与患者的ESR或CRP相关，19种菌群与ASDAS或BASDAI相关(P < 0.05)。AS患者口腔中fusobacteriae_unclassified与ESR、ASDAS-ESR呈正相关，且AS患者口腔中fusobacteriae_unclassified丰度高于健康志愿者。结论：强直性脊柱炎患者存在口腔菌群紊乱的现象，且菌群紊乱与疾病活动具有一定相关性。

Abstract: Objective: To investigate the relationship of oral flora disorder in ankylosing spondylitis by analyzing the differences of oral flora among ankylosing spondylitis patients and healthy controls, and to study the correlation between oral flora disorder and disease activity in ankylosing spondylitis. Methods: 66 ankylosing spondylitis patients and 31 healthy controls were enrolled in the study. The non-irritating saliva of all subjects was collected to extract DNA for 16SrRNA high-throughput sequencing using Illumina Miseq platform. The oral flora composition of all subjects was detected and analyzed to look for the differences between ankylosing spondylitis patients and healthy controls. The correlation between oral flora disorder and disease activity in ankylosing spondylitis is analyzed. Result: Results found that it consisted of 17 Phylums, 32 classes, 63 orders, 115 families, and 224 Genus in the oral cavity of all subjects in our study. The results from ɑ diversity analysis showed that the abundance and diversity of oral flora in ankylosing spondylitis patients group were greater than those in healthy group (P < 0.05). Although there was a certain difference of the composition of the oral flora between ankylosing spondylitis patients group and the healthy group, there was no statistic difference in β diversity (P > 0.05). Compared with healthy control group, the difference analysis showed that there was 1 species in the Phylums level, 3 species in the family level, 11 species in the genus level of bacteria different between AS patients and healthy volunteers (P < 0.05). Correlation analysis showed that there are 29 species associated with ESR or CRP in patients, 19 species are associated with ASDAS or BASDAI, (P < 0.05). The Fusobacteriaceae_unclassified in the oral cavity of AS patients was positively correlated with ESR and ASDAS-ESR, and the abundance of Fusobacteriaceae_unclassified in AS oral cavity was higher than that in normal subjects. Conclusions: There is a disorder in the oral flora of patients with ankylosing spondylitis. The disorder of oral flora was associated with disease activity.