对胰蛋白酶敏感性不同的口腔黏膜角化细胞活性的探讨

Study on the Activity of Keratinocytes in Oral Mucosa with Different Sensitivity to Trypsin

摘要: 目的：探讨对胰蛋白酶敏感性不同的口腔黏膜角化细胞在体外培养过程中活性的不同，筛选最具增殖能力的口腔黏膜角化细胞。方法：体外培养口腔黏膜角化细胞，传代过程中使用0.25%胰蛋白酶消化细胞，针对作用时间在4 min、5 min、6 min后脱离培养皿的细胞进行收集后分为A组、B组、C组，分别进行传代培养，通过显微镜观察细胞形态并计算细胞集落数；用CCK8法检测细胞的增殖能力。结果：B组最快到达生长对数期，细胞增殖能力最强，且细胞集落形成率最高，与其他组比较P < 0.05，差异具有统计学意义。结论：在口腔黏膜角化细胞传代过程中，0.25%胰蛋白酶消化时间5 min后进行接种培养可获得活性较强的角化细胞。

Abstract: Objective: To investigate the different activities of oral mucosa keratinocytes with different sensitivity to trypsin in vitro culture, and to screen the oral mucosa keratinocytes with the most proliferative ability. Methods: Oral mucosa keratinocytes were cultured in vitro. During the passage, 0.25% trypsin was used to digest the cells. The cells separated from the culture plate after 4 min, 5 min and 6 min were collected and divided into groups A, B and C. The proliferation ability of cells was measured by CCK8 method. Results: Group B reached the logarithmic stage of growth the fastest, the cell proliferation ability was the strongest, and the cell colony formation rate was the highest, compared with other groups, P < 0.05, the difference was statistically significant. Conclusion: During the passage of oral mucosa keratinocytes, the active keratinocytes could be obtained by inoculation and culture after 0.25% trypsin digestion for 5 min.