酚–胺表面化学构建一氧化氮催化涂层

Phenol-Amine Surface Chemical for Creating Nitric Oxide-Generating Coatings

摘要: 一氧化氮(NO)作为内皮功能关联因子，因其具有多重生物学功能，被广泛应用于多学科领域。发展先进的表面涂层技术固定NO催化功能分子是构建NO-催化型材料的常用手段。然而，材料表面实现功能分子的修饰往往涉及到复杂的表面预处理工艺以及功能分子接枝所需的多重步骤和昂贵试剂。因此，本研究发展“酚–胺”表面化学，选用具有邻苯二酚结构的酚类化学物质和具有类NO催化活性的分子胱胺(CySA)为涂层制备原材料，在弱碱性有氧的简单水溶液体系，基于“酚–胺”化学交联反应，通过“一步浸涂”法在材料表面构建以双硫键为NO催化活性中心的聚合粘附涂。该涂层通过催化内源性NO供体亚硝基硫醇实现NO持续释放，从而赋予材料抗凝、抑制平滑肌细胞增殖和促进内皮细胞生长的多重生物学功能。该简单、低成本、绿色的NO-催化型表面涂层方法显示出在血液接触类植入/介入器械表面改性上的广阔应用前景。

Abstract: Nitric oxide (NO), as a correlative factor of endothelial function, has been widely used in multidis-ciplinary fields due to its multiple biological functions. The development of advanced surface coating technology to immobilize NO catalytic functional molecules is a common means to construct NO-catalytic materials. However, the modification of functional molecules on the surface of materials often involves complex surface pretreatment processes, multiple steps and expensive reagents required for graft of functional molecules. Therefore, this research developed “phenol-amine” surface chemistry, chose phenol with catechol with class NO catalytic activity and the chemical molecules in (CySA) as the coating preparation of raw materials, in weak alkaline aerobic simple aqueous solution system, based on the chemical crosslinking reaction, through the “step dip-coating” method in material surface building with double sulphur keys to NO catalytic activity center of polymer adhesive coating. The coating catalyzes the continuous release of NO through the endogenous nitrite mercaptan of NO donor, thus endowing the material with multiple biological functions of anticoagulation, inhibition of smooth muscle cell proliferation and promotion of endothelial cell growth. This simple, low-cost, green NO-catalyzed surface coating method shows a broad application prospect in the surface modification of blood contact implants/interventional devices.