基于生物信息学分析DFNA5基因在肝癌中的表达及其与预后的关系

Analysis of DFNA5 Gene Expression in Liver Cancer and Its Relationship with Prognosis Based on Bioinformatics

摘要: 目的：对DFNA5基因与肝癌的发生发展及临床治疗预后之间的关系进行分析及预测。方法：通过HPA、TCGA、Oncomine、CCLE和Kaplan Meier-plotter数据库，分析DFNA5基因在人体组织中的表达分布及其在肝癌中的表达，探讨DFNA5与生存预后的关系。结果：DFNA5在正常人体中的分布没有显著组织特异性，在肝脏中的表达量略高于中位值；DFNA5蛋白主要分布于细胞质基质中，其蛋白表达量在甲状腺肿瘤中为最高，在肝癌中的表达水平则位于17种常见肿瘤组织中的第6位；核酸表达水平则是在神经胶质瘤中最高，在肝癌中的RNA表达水平与大多数肿瘤类型无显著区别。生存分析中，DFNA5基因与患者的生存时间表现为负相关(P = 0.002)，且与患者的性别分布有关，DFNA5的表达水平对于女性患者的生存预后有显著影响(P = 0.00013)，但对于男性患者的影响不显著(P = 0.051)。结论：DFNA5在正常机体中的表达分布没有明显组织特异性；在不同肿瘤组织中表达状况不一，在肝癌组织中的表达水平要高于癌旁组织；蛋白分布定位于细胞质基质，其高表达与肝癌患者的不良生存预后有关。

Abstract: Objective: To analyze the relationship between DFNA5 gene and the occurrence and development of liver cancer and predict the prognosis of clinical treatment. Methods: Through HPA, TCGA, Oncomine, CCLE, and Kaplan Meier-plotter, to analyze the expression distribution of DFNA5 gene in normal human tissues and its expression in liver cancer, the relationship between DFNA5 and survival prognosis was analyzed and discussed. Results: The distribution of DFNA5 in humans has no significant tissue specificity, and its expression in the liver is slightly higher than the median value; the protein is mainly distributed in the cytoplasmic matrix, and its protein expression is the highest in thyroid tumor, and its expression level in liver cancer is located at the 6th position among 17 general tumor tissues; the nucleic acid expression level is the highest in glioma cancer; the expression level of RNA in liver cancer is not significantly different from most tumor types. Survival analysis showed that the DFNA5 gene was negatively correlated with the patient's survival time (P = 0.002) and was related to the gender distribution of the patients. The expression level of DFNA5 had a significant impact on the survival prognosis of female patients (P = 0.00013), and effection on male patients was not significant (P = 0.051). Conclusion: The expression distribution of DFNA5 in healthy organisms has no tissue specificity; the expression status in different tumor tissues is distinct, and the expression level in liver cancer tissues is higher than that in adjacent tissues; the protein distribution is located in the cytoplasmic matrix, and its expression is high. It is related to the poor survival prognosis of liver cancer patients.