油茶叶提取物对金黄地鼠皮脂分泌的影响及其作用机制
Effect of Camellia oleifera Leaves Extract on the Development of Sebum Secretion in Golden Hamsters and Its Mechanism
DOI: 10.12677/HJFNS.2021.102015, PDF,   
作者: 崔心禹, 夏 琛, 刘 尧, 王璐馨, 吴晓琴, 沈建福：浙江大学生物系统工程与食品科学学院，浙江 杭州；张建军：江山之间生物科技有限公司，浙江 江山
关键词: 油茶叶提取物；皮脂腺；雄性激素；5α-还原酶；固醇调节元件结合蛋白； Camellia oleifera Leaves Extract； Sebaceous Gland； Androgen； 5α-Reductase； Sterol Regulatory Element Binding Protein
摘要: 目的：探讨油茶叶提取物(Camellia oleifera Leaves Extract, COLE)对金黄地鼠皮脂分泌的影响及其作用机制。方法：以金黄地鼠皮脂腺斑为研究载体，设置COLE低、高剂量组，空白对照组，丹参酮胶囊阳性对照组，进行为期28天灌胃实验。观察不同时间段COLE对金黄地鼠皮脂腺斑外观、显微结构、皮脂分泌的影响，测定比较各组血清、皮脂腺斑组织中脂质含量和雄性激素含量，最后通过研究SRD5A1/AR/ SREBP-1c信号通路探究COLE物抑制皮脂腺以及皮脂分泌的作用机制。结果：与空白对照组比较，COLE能够减小皮脂腺面积使其颜色变浅，显著缩小皮脂斑面积(p < 0.05)。HE染色结果显示COLE能够抑制皮脂腺的生长。油红O染色以及游离脂肪酸、甘油三酯、总胆固醇结果显示COLE显著能降低油脂的分泌水平。雄激素测定结果显示COLE能极显著降低金黄地鼠血清以及皮脂腺斑组织中双氢睾酮含量。Western blot实验结果证明COLE能够降低SRD5A1、AR、SREBP-1c蛋白表达的水平。结论：COLE通过抑制SRD5A1/AR/SREBP1c信号通路降低金黄地鼠皮脂腺脂质分泌，限制皮脂腺的发育进而改善皮脂腺斑的性状。
Abstract: Objective: To investigate the effect of Camellia oleifera Leaves Extract (COLE) on the sebum secretion in golden hamsters and its mechanism. Method: Using golden hamster sebaceous gland spots as the research carrier, the low and high dose groups of COLE, the blank control group, and the tanshinone capsule positive control group were set up for a 28-day gavage experiment. Observe the effects of COLE on the appearance, microstructure, and sebum secretion of the sebaceous plaques in golden hamsters at different time periods, then measure and compare the lipid content and androgen content in the serum, sebaceous plaque tissues of each group, and finally through the study of SRD5A1/AR/SREBP-1c signaling pathway explores the mechanism of COLE substances inhibiting sebaceous glands and sebum secretion. Results: Compared with the blank control group, COLE can reduce the area of sebaceous glands and make the color lighter. HE staining results show that COLE can inhibit the growth of sebaceous glands. Oil red O staining and the results of free fatty acids, triglycerides, and total cholesterol show that COLE can reduce the level of oil secretion. Androgen results show that COLE can significantly reduce the content of dihydrotestosterone in golden hamster serum and sebaceous plaque tissue. The results of Western blot experiments proved that COLE can reduce the expression levels of SRD5A1, AR, and SREBP-1c proteins. Conclusion: COLE inhibits the SRD5A1/AR/SREBP1c signaling pathway to reduce lipid secretion in the sebaceous glands of golden hamsters, restrict the development of sebaceous glands and improve the traits of sebaceous gland plaques.
文章引用：崔心禹, 夏琛, 刘尧, 王璐馨, 吴晓琴, 张建军, 沈建福. 油茶叶提取物对金黄地鼠皮脂分泌的影响及其作用机制[J]. 食品与营养科学, 2021, 10(2): 114-124. https://doi.org/10.12677/HJFNS.2021.102015

参考文献

[1] (2021) The Burden of Acne across the World. British Journal of Dermatology, 184, e37-e37. [Google Scholar] [CrossRef] 
[2] 耿雯瑾, 刘彤云. 儿童期痤疮[J]. 中华皮肤科杂志, 2021, 54(3): 273-275.
[3] Duru, P. and Örsal, Ö. (2021) The Effect of Acne on Quality of Life, Social Appearance Anxiety, and Use of Conventional, Complementary, and Alternative Treatments. Complementary Therapies in Medicine, 56, Article ID: 102614. [Google Scholar] [CrossRef] [PubMed]
[4] Bansal, P., Sardana, K., Vats, G., Sharma, L., Garga, U. and Khurana, A. (2020) A Prospective Study Examining Trigger Factors and Hormonal Abnormalities in Adult Female Acne. Indian Dermatology Online Journal, 11, 544-550.
[5] 单雨婷, 路永红, 杜秀君. 雄激素及雄激素受体在痤疮发病中的作用[J]. 皮肤病与性病, 2016, 38(2): 108-112.
[6] Diane, T., Kathryn, G., Jan, L. and Donald, L. (1999) An-drogen Metabolism in Sebaceous Glands From Subjects with and without Acne. Archives of Dermatology, 135, 1041-1045. [Google Scholar] [CrossRef] [PubMed]
[7] Thiboutot, D. (2004) Regulation of Human Seba-ceous Glands. Journal of Investigative Dermatology, 123, 1-12. [Google Scholar] [CrossRef] [PubMed]
[8] Avila, D.M., Fuqua, S.A., George, F.W. and McPhaul, M.J. (1998) Identification of Genes Expressed in the Rat prostate That Are Modulated Differently by Castration and Fi-nasteride Treatment. The Journal of Endocrinology, 159, 403-411. [Google Scholar] [CrossRef] [PubMed]
[9] Nantermet Pascale, V., Xu, J., Yu, Y., Hodor, P., Holder, D., Adamski, S., et al. (2004) Identification of Genetic Pathways Activated by the Androgen Receptor during the Induction of Prolifer-ation in the Ventral Prostate Gland. The Journal of Biological Chemistry, 279, 1310-1322. [Google Scholar] [CrossRef] 
[10] Rosignoli, C., Nicolas, J.C., Jomard, A. and Michel, S. (2003) In-volvement of the SREBP Pathway in the Mode of Action of Androgens in Sebaceous Glands in Vivo. Experimental Dermatology, 12, 480-489. [Google Scholar] [CrossRef] [PubMed]
[11] Zhu, L., Du, W., Liu, Y.P., Cheng, M.J., Wang, X.M., Zhang, C.H., et al. (2018) Prolonged High-Glucose Exposure Decreased SREBP-1/FASN/ACC in Schwann Cells of Diabetic Mice via Blocking PI3K/Akt Pathway. Journal of Cellular Biochemistry, 120, 5777-5789. [Google Scholar] [CrossRef] [PubMed]
[12] Zhang, D., Tomisato, W., Su, L., Sun, L., Choi, J.H., Zhang, Z., et al. (2017) Skin-Specific Regulation of SREBP Processing and Lipid Biosynthesis by Glycerol Kinase 5. Proceedings of the National Academy of Sciences of United States of America, 114, E5197-E5206. [Google Scholar] [CrossRef] [PubMed]
[13] 胡彬, 杨逸璇, 杨婉婷, 王峰, 陶梦颖, 朱明芳. 不同浓度的石榴皮多酚软膏对兔耳粉刺模型组织病理形态学的影响[J]. 湖南中医药大学学报, 2018, 38(4): 397-401.
[14] 邱稼飞, 王璇, 张南海, 赵亮, 张列兵, 吴薇, 籍保平, 周峰. 佛手和甜橙果汁及挥发油对油酸致兔耳痤疮的预防作用[J]. 中国农业大学学报, 2020, 25(11): 82-89.
[15] 张媛, 张荣平, 张志毕, 杨晖, 杨丽晗, 王元, 唐莹. 龙血竭凝胶对兔耳痤疮模型TNF-α、IL-6和MFGE8的影响[J]. 中国皮肤性病学杂志, 2021, 35(1): 30-36.
[16] 魏伟. 药理实验方法学[M]. 第4版. 北京: 人民卫生出版社, 2010.
[17] 吴昊. 药根碱对HepG2细胞和金黄地鼠的降血脂活性及机制研究[D]: [硕士学位论文]. 重庆: 西南大学, 2014: 1-79.
[18] 王微. 香水莲花提取物改善阿尔兹海默症学习记忆能力的作用研究[D]: [硕士学位论文]. 杭州: 浙江大学, 2019: 1-95.