不同厂家MRS培养基质量比较

Compari-son Study on Quality of MRS Agar from Different Manufacturers

摘要: 目的：比较不同厂家的MRS培养基和莫匹罗星锂盐改良的MRS培养基的质量。方法：分别对三个厂家的MRS培养基和莫匹罗星锂盐改良的MRS培养基的理化特性、菌株的生长能力及抑菌能力进行测试。结果：三个厂家的MRS培养基、莫匹罗星锂盐改良的MRS培养基的pH值差异不大。在生长能力方面，各测试菌株均能在厂家I和II的两种培养基上正常生长。但是对于厂家III，在MRS培养基上嗜酸乳杆菌As1.1878在48 h内不能生长，三个厂家的生长菌落数(除乳双歧杆菌ATCC118004外)的差异具有统计学意义(P < 0.05)；在改良MRS培养基上，有两株双歧杆菌48 h不能生长，生物学效果差异有显著性(P < 0.05)。在菌落大小方面，厂家I优于厂家II和厂家III。在抑菌能力方面，三个厂家的改良MRS培养基性能相当。结论：综合考虑测试的结果，厂家I的培养基质量优于厂家II和厂家III。

Abstract: Object: To compare the quality of MRS medium and mupiroxacin lithium modified MRS medium from different manufacturers. Methods: Testing the chemical properties, growth ability and selectivity of MRS medium and mupiroxacin lithium modified MRS medium. Results: There was no significant difference in the pH value of MRS medium and the modified MRS medium between the three manufacturers. In terms of growth ability, all tested strains could grow normally on two kinds of media of manufacturer I and II. However, for manufacturer III, Lactobacillus acidophilusAs1.1878 could not grow within 48 hours on MRS medium, and the difference of colony count among the three manufacturers (except for Bifidobacterium lactis ATCC 118004) was statistically significant (P < 0.05); On the modified MRS medium, two Bifidobacterium strains could not grow for 48 hours, and the biological effects were significantly different (P < 0.05). The target colony size of manufacturer I is better than that of manufacturer II and III. In terms of selectivity, the performance of the modified MRS medium from the three manufacturers was comparable. Conclusion: Considering the test results, the quality of culture medium of manufacturer I is better than that of manufacturer II and III.