富含脂肪酶菌种的筛选与鉴定

Screening and Identification of Lipase-Rich Strains

摘要: 脂肪酶是生物柴油生产的关键酶，为了降低生物柴油的研发成本，本研究从餐厅油脂污染处和自然环境中筛选得到了含脂肪酶菌；然后对其进行了紫外以及高温诱变。对诱变后的脂肪分解菌利用罗丹明B固体培养基对其活性进行了鉴定；利用p-NP法测定了活性；筛选得到了相对活性最高的脂肪分解菌，最后，通过16 s测序鉴定了富含脂肪酶菌的菌种或菌属，为进一步生物柴油的开发提供了实验基础。

Abstract: Lipase is the key enzyme in biodiesel production. In order to reduce the development cost of bio-diesel, lipase-rich strains were screened from the restaurant grease contamination and natural environment, and then the lipase-rich strains were mutagenized by UV and high temperature. The activity of the mutated lipolytic bacteria was identified by using Rhodamine B solid medium, and determined by P-NP method. Finally, the species or genus of lipase-rich strains were identified by 16 S sequencing, which provided an experimental basis for the further development of biodiesel.