大洋河刀鲚鳃转录组测序与分析
Transcriptome Sequencing and Analysis for Gills of Coilia nasus from Dayang River
DOI: 10.12677/OJFR.2021.84021, PDF,    科研立项经费支持
作者: 张伯序, 胡宗云, 张 健, 王建军, 杨培民*：辽宁省淡水水产科学研究院，辽宁省水生动物病害防控重点实验室，辽宁 辽阳
关键词: 大洋河；刀鲚；转录组测序；Dayang River； Coilia nasus； RNA-Seq Sequencing
摘要: 为探究盐度影响与刀鲚(Coilia nasus)腮组织转录组的关系，本实验对取自大洋河流域不同盐度水体的刀鲚的腮组织进行了转录组测序和分析。结果显示：一共有70,964条unigenes被注释；刀鲚的unigene序列与大西洋鲱(Clupea harengus)最为接近；有关细胞进程、生物调节、代谢过程的unigenes占比很高；腮组织部有关代谢功能的转录组表达十分旺盛；生物体系统(Organismal Systems)与环境信息处理的信号通路(Environmental Information Processing)的unigene数最多。
Abstract: In order to explore the relationship between salinity effects and transcriptome of (Coilia nasus) gill tissues, transcriptome sequencing and analysis were performed on the gill tissues of Coilia nasus from waters with different salinity in the Dayang River basin. The results showed that 70964 unigenes were annotated. The unigene sequence of Coilia nasus was closest to that of Atlantic Herring (Clupea harengus). The proportion of unigenes related to cell process, biological regulation and metabolic process was high. Transcriptome expression related to metabolic function was very vigorous in the gill tissue. Organismal Systems and Environmental Information Processing have the largest number of unigene.
文章引用：张伯序, 胡宗云, 张健, 王建军, 杨培民. 大洋河刀鲚鳃转录组测序与分析[J]. 水产研究, 2021, 8(4): 175-184. https://doi.org/10.12677/OJFR.2021.84021

参考文献

[1] 解玉浩. 东北地区淡水鱼类[M]. 沈阳: 辽宁科学技术出版社, 2007, 148.
[2] Liu, D., Li, Y.Y., Tang, W.Q., et al. (2014) Population Structure of Coilia nasus in the Yangtze River Revealed by Insertion of Short Interspersed Elements. Biochemical Systematics and Ecology, 54, 103-112.
[Google Scholar] [CrossRef] 
[3] 张健, 杨培民, 胡宗云, 等. 大洋河刀鲚繁殖生物学特性[J]. 淡水渔业, 2021, 51(6): 3-11.
[4] Hata, H. (2018) The IUCN Red List of Threatened Species. Page Bros, Norwich.
[5] 程方圆, 陶紫玉, 李晨虹. 应用单核苷酸多态性(SNP)标记鉴定短颌鲚､湖鲚和刀鲚[J]. 上海海洋大学学报, 2019, 28(1): 10-19.
[6] 王生, 方春林, 周辉明, 等. 鄱阳湖刀鲚的渔汛特征及渔获物分析[J]. 水生态学杂志, 2017, 38(6): 82-87.
[7] 施永海, 张根玉, 张海明, 等. 刀鲚的全人工繁殖及胚胎发育[J]. 上海海洋大学学报, 2015, 24(1): 36-43.
[8] 杨巧莉. 中国鱿属鱼类进化关系及刀鳞、凤鱿的分子系统地理学研究[D]: [博士学位论文]. 青岛: 中国海洋大学, 2012.
[9] Zhu, G., Wang, L., Tang, W., et al. (2014) De Novo Transcriptomes of Olfactory Epithelium Reveal the Genes and Pathways for Spawning Migrationin Japanese Grenadier Anchovy (Coilia nasus). PLoS ONE, 9, e103832.
[Google Scholar] [CrossRef] [PubMed]
[10] Li, S., Shen, L., Sun, L., et al. (2017) Small RNA-Seq Analysis Reveals microRNA-Regulation of the Imd Pathway during Escherichia coli Infection in Drosophila. Developmental & Comparative Immunology, 70, 80-87.
[Google Scholar] [CrossRef] [PubMed]
[11] Jia, Z., Wang, Q., Wu, K., et al. (2017) De Novo Transcriptome Sequencing and Comparative Analysis to Discover Genes Involved in Ovarian Maturity in Strongylocentrotus nudus. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 23, 27-38.
[Google Scholar] [CrossRef] [PubMed]
[12] Pelis, R.M. and McCormick, S.D. (2001) Effects of Growth Hormone and Cortisol on Na+-K+-2Cl- Cotransporter Localization and Abundance in the Gills of Atlantic Salmon. General and Comparative Endocrinology, 124, 134-143.
[Google Scholar] [CrossRef] [PubMed]
[13] 王艳, 胡先成. 不同盐度下鲈鱼稚鱼鳃的显微结构观察[J]. 海洋科学, 2009, 33(12): 138-142.
[14] Heijden, A., Verbost, P., Eygenstcyn, J., et al. (1997) Mitochondria-Rich Cells in Gills of Tilapia (Oreochro mismossambicus) Adapted to Fresh Water or Sea Water: Quantification by Confocal Laser Scanning Microscopy. Journal of Experimental Biology, 200, 55-64.
[Google Scholar] [CrossRef] [PubMed]
[15] Dang, Y., Xu, X., Shen, Y., et al. (2016) Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila. PLoS ONE, 11, e0157413.
[Google Scholar] [CrossRef] [PubMed]
[16] Pereiro, P., Balseiro, P., Romero, A., et al. (2012) High-Throughput Sequence Analysis of Turbot (Scophthalmus maximus) Transcriptome Using 454-Pyrosequencing for the Discovery of Antiviral Immune Genes. PLoS ONE, 7, e35369.
[Google Scholar] [CrossRef] [PubMed]
[17] Sutherland, B.J., Koczka, K.W., Yasuike, M., et al. (2014) Comparative Transcriptomics of Atlantic Salmo salar, Chum Oncorhynchus keta and Pink Salmon O. gorbuscha during Infections with Salmon Lice Lepeophtheirus salmonis. BMC Genomics, 15, Article No. 200.
[Google Scholar] [CrossRef] [PubMed]

为你推荐