HPLC法同时测定苗药芭蕉根、茎、叶中羽扇豆酮和豆甾醇的含量

Determination of Lupanone and Stigmasterol in the Roots, Stems and Leaves of Musa japonica by HPLC

摘要: 目的：比较苗药芭蕉根、茎、叶中羽扇豆酮及豆甾醇的含量，为该药材的资源开发利用提供有力依据。方法：采用高效液相色谱法(HPLC)；色谱柱：Diamonsil-C₁₈ (4.6 mm × 250 mm, 5 µm)；流动相：甲醇–乙腈(体积比50:50)，等度洗脱；检测波长：206 nm；流速：0.8 ml/min；柱温：30℃；进样量：10 μl。采用SPSS 26.0、SIMCA 11.5统计软件对含量测定结果进行统计学分析、聚类分析、主成分分析。结果：羽扇豆酮和豆甾醇的质量浓度分别在6.26 ~150.30 µg/ml、11.26~270.20 µg/ml范围内与各自峰面积成良好的线性关系；平均回收率均分别在95%~105%之间；14批芭蕉根与茎中羽扇豆酮的含量为94.46~917.23 µg/g、180.82~1667.86 µg/g，豆甾醇的含量117.27~315.08 µg/g、271.26~1186.74 µg/g。统计学分析结果表明，同一植株芭蕉药材不同部位的羽扇豆酮和豆甾醇的含量有统计学差异(P < 0.05)，芭蕉叶中未检测出；主成分分析与聚类分析结果均与统计学分析结果一致。结论：研究结果表明，芭蕉茎的含量大于芭蕉根的含量，为了更好地利用药物资源，芭蕉茎可作为羽扇豆酮和豆甾醇的原植物材料来源。

Abstract: Objective: To compare the content of lupanone and stigmasterol in the root, stem and leaf of Plan-tain plantain, and to provide a strong basis for the resource development and utilization of plantain. Methods: HPLC was used. Diamonsil-C₁₈ (4.6 mm × 250 mm, 5 µm) column; Mobile phase: metha-nol-acetonitrile (50:50 by volume), isometric elution; Detection wavelength: 206 nm; Flow rate: 0.8 ml/min; Column temperature: 30˚C; Injection volume: 10 μL. SPSS 26.0 and SIMCA 11.5 were used to analyze the contents of lupanone and stigmasterol in plantain roots, stems and leaves by statisti-cal analysis, cluster analysis and principal component analysis. Results: The concentrations of lu-panone and stigmasterol had a good linear relationship with the peak areas in the range of 6.26~150.30 µg/ml and 11.26~270.20 µg/ml, respectively. The average recoveries were 95%~105%. The contents of lupanone in the roots and stems of 14 batches of Plantains were 94.46~917.23 µg/g and 180.82~1667.86 µg/g, and the contents of stigmasterol were 117.27~315.08 µg/g and 271.26~1186.74 µg/g. The results of statistical analysis showed that there were significant differences in the contents of lupanone and stigmasterol in different parts of M. japonica from the same plant (P < 0.05), but no significant differences were detected in M. japon-ica leaves. The results of principal component analysis and cluster analysis were consistent with those of statistical analysis. Conclusion: The results showed that the content of banana stem was higher than that of banana root. In order to make better use of drug resources, banana stem could be used as the source of lupanone and stigmasterol.