Gli1和Shh在卵巢子宫内膜异位症在位和异位子宫内膜的表达及意义
Expression and Significance of Gli1 and Shh in Eutopic and Ectopic Endometrium of Ovarian Endometriosis
DOI: 10.12677/ACM.2022.124392, PDF,   
作者: 王进叶：青岛大学附属医院妇科，山东 青岛；青岛市市南区八大湖街道巢湖路社区卫生服务中心，山东 青岛；赵 飞, 王黎明*：青岛大学附属医院妇科，山东 青岛
关键词: 卵巢子宫内膜异位症；Gli1；Shh；复发；Endometriosis； Gli1； Shh； Recrudescence
摘要: 目的：探讨胶质瘤相关癌基因同源物1 (Gli1)和超音刺猬因子(Shh)在卵巢子宫内膜异位症(EMT)在位和异位子宫内膜的表达及意义。方法：选取青岛大学附属医院2018年1月至2019年10月手术的50例EMT患者的在位子宫内膜(EMT1组)和异位子宫内膜(EMT2组)，同期50例正常子宫内膜(对照组)，实时荧光定量聚合酶链反应(qRT-PCR)法和免疫组化法检测三组内膜Gli1、Shh mRNA和蛋白的表达，分析EMT组织中Gli1与Shh表达相关性，及Gli1和Shh蛋白表达与EMT患者临床分期及术后复发的关系。结果：EMT在位和异位子宫内膜Gli1、Shh mRNA和蛋白表达均高于对照组(P < 0.05)。EMT组织中，Gli1与Shh mRNA、蛋白表达均呈正相关(r = 0.621; r = 0.637, P < 0.05)，临床分期III~IV期EMT患者Gli1和Shh蛋白高表达率高于I~II期患者(P < 0.05)。手术年龄轻、Gli1高表达是EMT患者术后复发的危险因素(P < 0.05)。结论：Gli1、Shh在EMT在位和异位子宫内膜表达上调，与EMT临床分期相关，Gli1、Shh可能参与了EMT的发生发展，Gli1高表达提示EMT患者术后复发风险高。
Abstract: Objective: To investigate the expression and significance of glioma associated oncogene homolog 1 (Gli1) and sonic hedgehog (Shh) in eutopic and ectopic endometrium of ovarian endometriosis (EMT). Methods: Eutopic endometrium (EMT group 1) and ectopic endometrium (EMT group 2) in 50 patients with EMT and the normal endometrium (control group) in 50 patients were selected from the Affiliated Hospital of Qingdao University from January 2018 to October 2019, the mRNA and protein expression levels of Gli1 and Shh were detected by qRT-PCR and IHC staining. The correlation between the expression of Gli1 and Shh in EMT tissue was analyzed, and the relationship between the expression of Gli1 and Shh protein and clinical stages and postoperative recurrence of EMT patients was analyzed. Results: The mRNA and protein expressions of Gli1 and Shh in eutopic and ectopic endometrium of EMT were higher than those in control group (P < 0.05). In EMT tissue，Gli1 was positively correlated with the mRNA and protein expression of Shh (r = 0.621; r = 0.637, P < 0.05); the expression of Gli1 and Shh protein was related to clinical stage of EMT patients (P < 0.05). Young operative age and Gli1 overexpression were the risk factors for postoperative recurrence in EMT patients (P < 0.05). Conclusion: Gli1 and Shh are up-regulated in eutopic and ectopic endometrium of EMT, which is related to the clinical stage of EMT. Gli1 and Shh may be involved in the occurrence and development of EMT. The high expression of Gli1 indicates a high risk of postoperative recurrence in EMT patients.
文章引用：王进叶, 赵飞, 王黎明. Gli1和Shh在卵巢子宫内膜异位症在位和异位子宫内膜的表达及意义[J]. 临床医学进展, 2022, 12(4): 2728-2734. https://doi.org/10.12677/ACM.2022.124392

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