基于网络药理学探讨灵芝治疗哮喘的作用机制

Study on the Mechanism of Ganoderma in Treating Asthma Based on Network Pharmacology

摘要: 目的：本研究采用网络药理学的方法探讨灵芝治疗哮喘的可能作用机制。方法：通过检索TCMSP数据库筛选灵芝的活性成分，在GeneCards数据库中查找哮喘的相关靶点，取交集基因进行蛋白互作分析，GO生物富集及KEGG富集分析。结果：得出灵芝活性成分14个，灵芝与哮喘的潜在相交靶标24个，主要有NR3C2、PGR、NCOA2等；基于DAVID数据库，GO功能分析与KEGG通路富集分析结果表明，灵芝参与的主要信号通路有adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway、response to estradiol和G-protein coupled acetylcholine receptor signaling pathway等。结论：灵芝可能是通过调控Apoptosis-multiple species、Hepatitis B、Cholinergic synapse和Human immunodeficiency virus 1 infection等信号通路上的CASP9，JUN，CASP8和CASP3等基因发挥治疗哮喘的作用。

Abstract: Objective: To explore the possible mechanism of Ganoderma in the treatment of asthma. Methods: The active components of Ganoderma were screened by searching the tcmsp database, and the related targets of asthma were found in the genecards database. The intersection genes were taken for protein interaction analysis, go bioaccumulation, and KEGG enrichment analysis. Results: There were 14 active ingredients in Ganoderma and 24 potential cross targets between Ganoderma and asthma, mainly NR3C2, PGR, NCOA2, etc; Based on David database, go function analysis and KEGG pathway enrichment analysis results show that the main signal pathways involved by Ganoderma include adenylate cycle inhibiting G-protein coupled acetylcholine receiver signaling pathway, response to estradiol, G-protein coupled acetylcholine receiver signaling pathway, etc. Conclusion: Ganoderma lucidum may play a role in the treatment of asthma by regulating CASP9, Jun, CASP8, and CASP3 genes on the signal pathways of apoptosis-multiple species, hepatitis B, cholinergic synapse, and human immunodeficiency virus 1 infection.