非小细胞肺癌A549和H520细胞PDCD4和AP-1表达与细胞迁移的相关性研究
Correlation between the Expression of PDCD4 and AP-1 and Cell Migration in Non-Small Cell Lung Cancer A549 and H520 Cells
DOI: 10.12677/ACM.2022.127941, PDF,    科研立项经费支持
作者: 钟 燕*, 何 琴*, 范苏苏, 朱钰珊, 张 旋#：昆明医科大学药学院暨云南省天然药物药理重点实验室，云南 昆明；王应霞#：昆明医科大学第一附属医院病理科，云南 昆明
关键词: 非小细胞肺癌；肺腺癌；肺鳞癌；程序性细胞死亡因子4；激活蛋白-1；迁移；Non Small Cell Lung Cancer； Lung Adenocarcinoma； Lung Squamous Cell Carcinoma； Programmed Cell Death Factor 4； Activator Protein-1； Migration
摘要: 目的：研究非小细胞肺癌A549和H520细胞PDCD4和AP-1 (JunB和c-Fos)表达及其与细胞迁移的关系。方法：体外培养非小细胞肺癌肺腺癌细胞A549和肺鳞癌细胞H520，细胞划痕实验检测细胞迁移情况，western blot方法检测细胞PDCD4和AP-1 (JunB和c-Fos)表达情况，比较肺腺癌细胞A549和肺鳞癌细胞H520中PDCD4和AP-1 (JunB和c-Fos)表达差异以及细胞迁移能力的差异。结果：肺腺癌细胞A549中PDCD4蛋白表达明显低于肺鳞癌细胞H520，而AP-1 (JunB和c-Fos)蛋白表达明显高于肺鳞癌细胞H520，且肺腺癌细胞A549的迁移能力明显高于肺鳞癌细胞H520，差异有统计学意义(P < 0.05)。结论：肺腺癌细胞PDCD4蛋白表达低于肺鳞癌细胞，AP-1 (JunB和c-Fos)蛋白表达高于肺鳞癌细胞，细胞迁移能力与PDCD4表达呈负相关，与AP-1表达呈正相关。
Abstract: Objective: To study the expression of PDCD4 and AP-1 (JunB and c-Fos) in non-small cell lung cancer A549 and H520 cells and their correlation with the migration ability of cells. Methods: Non- small cell lung cancer cells-lung adenocarcinoma cells A549 and lung squamous cell carcinoma cells H520 were cultured in vitro. Cell migration was detected by cell scratch test, and the expression of PDCD4 and AP-1 (JunB and c-Fos) was detected by Western blot. The expression difference of PDCD4 and AP-1 (JunB and c-Fos) and the difference of cell migration rate between lung adenocarcinoma cell A549 and lung squamous cell carcinoma cell H520 were compared. Results: The expression of PDCD4 protein in lung adenocarcinoma cell line A549 was significantly lower than that in lung squamous cell carcinoma cell line H520, while the expression of AP-1 (JunB and c-Fos) protein was significantly higher than that in lung squamous cell carcinoma cell line H520, and the migration ability of lung adenocarcinoma cell line A549 was significantly higher than that of lung squamous cell carcinoma cell line H520 (P < 0.05). Conclusions: The expression of PDCD4 protein in lung ade-nocarcinoma cells is lower than that in lung squamous cell carcinoma cells, and the expression of AP-1 (JunB and c-Fos) protein is higher than that in lung squamous cell carcinoma cells. The cell migration ability is negatively correlated with the expression of PDCD4 and positively correlated with the expression of AP-1.
文章引用：钟燕, 何琴, 范苏苏, 朱钰珊, 王应霞, 张旋. 非小细胞肺癌A549和H520细胞PDCD4和AP-1表达与细胞迁移的相关性研究[J]. 临床医学进展, 2022, 12(7): 6517-6524. https://doi.org/10.12677/ACM.2022.127941

参考文献

[1] Zhang, Y., Ren, J.S., Huang, H.Y., Shi, J.F., Li, N., Zhang, Y., et al. (2018) International Trends in Lung Cancer Inci-dence from 1973 to 2007. Cancer Medicine, 7, 1479-1489. [Google Scholar] [CrossRef] [PubMed]
[2] 苏艳云, 邵月山. 非小细胞肺癌靶向治疗的研究进展[J]. 中国城乡企业卫生, 2022, 37(3): 55-57.
[3] Cao, W.B., Wei, M., Li, B., Liu, Y., Lu, Y., Tang, Z., et al. (2016) Functional Role of Eukaryotic Translation Initiation Factor 4 γ1(EIF4G1) in NSCLC. Oncotarget, 7, 24242-24251. [Google Scholar] [CrossRef] [PubMed]
[4] Ye, Z.C., Huang, Y.M., Ke, J., Zhu, X., Leng, S. and Luo, H. (2021) Breakthrough in Targeted Therapy for Non- Small-Cell Lung Cancer. Biomedi-cine & Pharmacotherapy, 133, Article ID: 111079. [Google Scholar] [CrossRef] [PubMed]
[5] Wu, Y., Zhu, X., Shen, R., Huang, J., Xu, X. and He, S. (2019) miR-182 Contributes to Celladhesion-Mediated Drug Resistancin Multiple Myeloma via Targeting PDCD4. Pa-thology - Research and Practice, 215, Article ID: 152603. [Google Scholar] [CrossRef] [PubMed]
[6] Hao, Y.J., Huang, J.W., Ma, Y., Chen, W., Fan, Q., Sun, X., et al. (2018) Asiatic Acid Inhibits Proliferation, Migration and Induces Apoptosis by Regulating Pdcd4 via the PI3K/Akt/mTOR/p70S6K Signaling Pathway in Human Colon Carcinoma Cells. Oncology Letters, 15, 8223-8230. [Google Scholar] [CrossRef] [PubMed]
[7] Rylski, M. and Kaczmarek, L. (2004) Ap-1 Targets in the Brain. Fron-tiers in Bioscience-Landmark, 9, 8-23. [Google Scholar] [CrossRef] [PubMed]
[8] Shaulian, E. and Karin, M. (2002) AP-1 as a Regulator of Cell Life and Death. Nature Cell Biology, 4, E131-E136. [Google Scholar] [CrossRef] [PubMed]
[9] Eferl, R. and Wagner, E.F. (2003) AP-1: A Double-Edged Sword in Tumorigenesis. Nature Reviews Cancer, 3, 859- 868. [Google Scholar] [CrossRef] [PubMed]
[10] Alder, H., Taccioli, C., Chen, H., Jiang, Y., Smalley, K.J., Fadda, P., et al. (2012) Dysregulation of miR-31 and miR- 21 Induced by Zinc Defi-ciency Promotes Esophageal Cancer. Carcinogenesis, 33, 1736-1744. [Google Scholar] [CrossRef] [PubMed]
[11] Fischer, N., Goke, F., Splittstosser, V., Lankat-Buttgereit, B., Müller, S.C. and Ellinger, J. (2012) Expression of Programmed Cell Death Protein 4 (PDCD4 ) and miR-21 in Urothelial Carci-noma. Biochemical and Biophysical Research Communications, 417, 29-34. [Google Scholar] [CrossRef] [PubMed]
[12] 朱潇鹏, 徐庆福, 吕胜青. miR-21与AP-1构成的自我调节环路在脑胶质瘤中的研究进展[J]. 国际神经病学神经外科学志, 2013, 40(4): 387-390.
[13] 林小峰, 陈龙. 晚期非小细胞肺癌化疗现状及进展[J]. 广西医科大学学报报, 2019, 36(5): 850-855.
[14] Santarpia, M., Liguori, A., Kara-chaliou, N., Gonzalez-Cao, M., Daffinà, M.G., D’Aveni, A., et al. (2017) Osimertinib in the Treatment of Non-Small-Cell Lung Cancer: Design, Development and Place in Therapy. Lung Cancer, 8, 109-125. [Google Scholar] [CrossRef] 
[15] 李明, 裴晓宁, 岳恺, 木亚林, 张成辉. miR-21靶向PDCD4调控非小细胞肺癌A549细胞的增殖与迁移[J]. 中国肿瘤生物治疗杂志, 2021, 28(4): 332-338.
[16] 罗麟洁, 王鸿程. JUN和FOS在非小细胞肺癌中的表达及其与STAT关系的研究进展[J]. 国际呼吸杂志, 2008, 28(13): 818-821.
[17] 陈娟, 毕明宏, 高振远, 王雅萍. COX-2和c-Fos在非小细胞肺癌中的表达及临床意义[J]. 山东第一医科大学(山东省医学科学院)学报, 2018, 39(11): 1248-1250.
[18] 张满, 特木热, 郭占林. 上皮间质转化(EMT)与肺癌的关系[J]. 内蒙古医学杂志, 2014, 46(12): 1472-1474.
[19] Wanna-Udom, S., Terashima, M., Lyu, H., Ishi-mura, A., Takino, T., Sakari, M., et al. (2020) The m6A Methyltransferase METTL3 Contributes to Transforming Growth Factor-Beta-Induced Epithelial-Mesenchymal Transition of Lung Cancer Cells through the Regulation of JUNB. Biochemical and Biophysical Research Communications, 524, 150-155. [Google Scholar] [CrossRef] [PubMed]

为你推荐