不同采收期滇鼠刺中Iteafuranal A的含量变化分析

Dynamic Content Analysis of Iteafuranal A in Itea yunnanensis at Different Harvest Periods

摘要: 目的：分析不同采收期滇鼠刺中Iteafuranal A的动态变化，为明确滇鼠刺的最佳采收期提供科学依据。方法：以ZORBAX SB-C18柱(5 µm, 4.6 * 250 mm)为色谱柱，以0.1%醋酸水–溶液(A)–甲醇(B)为流动相进行梯度洗脱；流速为1.0 mL/min；检测波长为306 nm；柱温为30℃。结果：在上述色谱条件下，Iteafuranal A在0.042~1.42 mg范围内与峰面积线性关系良好，R² = 0.9981 (n = 6)；平均加样回收率为100.4%，RSD值为1.02% (n = 6)；6批不同采收期滇鼠刺中Iteafuranal A的含量分别为：0.088%、0.121%、0.263%、0.132%、0.084%以及0.032%。结论：不同采收期滇鼠刺中Iteafuranal A的含量具有显著差异，并初步确定1月份为滇鼠刺的最佳采收期。

Abstract: Objective: The dynamic content change of Iteafuranal A was analyzed in Itea yunnanensis collected at different harvest periods, in order to provide the scientific basis for the best harvest time determination. Methods: A HPLC method was established based on the ZORBAXSB-C18 column (5 µm, 250 mm * 4.6 mm) eluted with a mobile phase of 0.1% acetic acid (A)-methanol (B) in gradient. The flow rate was 1.0 mL/min and the detection wavelength at 306 nm as the well as column temperature at 30˚C. Results: The calibration curve was linear within the range of 0.042~1.42 mg of Iteafuranal A (R² = 0.9981), the average recovery rate was 100.4% with the RSD value of 1.02% (n = 6). The content of Iteafuranal A in I. yunnanensis at six different harvest times was determined as 0.088%, 0.121%, 0.263%, 0.132%, 0.084% and 0.032%, respectively. Conclusion: There was a significant difference in the content of Iteafuranal A in the 6 batches of samples, and January was ini-tially identified as the best harvest period.