葡萄糖溶液标准物质的研制

Development of Reference Materials for Glucose Aqueous Solution

摘要: 针对食品中糖类检测的需求以及国内缺乏葡萄糖单一组分水溶液标准物质的现状，研制了浓度分别为2.78 mmol/L、5.56 mmol/L、11.11 mmol/L的葡萄糖溶液标准物质。采用重量称量法配制，根据葡萄糖的纯度、称取葡萄糖的质量以及定容体积来确定其特性量值，对其均匀性和稳定性进行了考察，并进行不确定度评定。结果表明，葡萄糖溶液标准物质的均匀性良好，能够在−70℃下稳定储存6个月。量值核验结果采用比对判据E_n值进行评价，比对结果满意，量值准确可靠。该标准物质有望于相关仪器的校准、葡萄糖的含量检测以及分析方法评价等领域得到广泛应用。

Abstract: To meet the needs of sugar detection in food and the lack of glucose aqueous solution reference materials for single component, glucose aqueous solution reference materials with the concentra-tions of 2.78 mmol/L, 5.56 mmol/L and 11.11 mmol/L were developed. The reference materials were prepared by weight-volume method. The certified value was determined according to the pu-rity and the weight of glucose and the solution volume. The uniformity and stability were investi-gated and the uncertainty was evaluated. The results showed that glucose aqueous solution refer-ence materials had favorable uniformity, and they can be stably stored at −70˚C for 6 months. The verification results were evaluated by the comparison criterion E_n value and the comparison results were satisfactory. The values are accurate and reliable. They are expected to be used in the calibra-tion of relevant instruments, the detection of glucose content and the evaluation of analytical methods.