一株产红色素真菌的分离鉴定与特性分析

Identification of a Red Pigment-Producing Filamentous Fungus and Characterization of Its Pigments

摘要: 从柚子皮壳上分离得到一株产红色素丝状真菌菌株RedF，通过形态学特性指标和基因组多位点序列分型法鉴定菌株为暗玫瑰篮状菌。镉离子对菌落形态建成影响在遗传上是可逆转的。水溶性红色素的最大吸收峰为503 nm。以察氏培养基为基础，添加0.3%羧甲基纤维素钠时，红色素产量最大；理化条件稳定性实验表明，红色素粗提液20℃~60℃条件下较稳定；低浓度(0.002 g/L)亚硫酸钠对红色素粗提液有增色效应；红色素在含H2O2微环境下稳定性下降；在NaCl盐度的增加时，红色素的稳定性下降。红色素粗提液对蛋白类、淀粉类食材物质的着色能力与稀释100倍的红曲色素相当。研究结果能为微生物红色素资源的开发利用提供参考。

Abstract: A red pigment producing fungal strain RedF was isolated from pomelo peel. The strain was identified to Talaromyces atroroseus depending on the morphology property indexes and genome multilocus sequence typing method. The effects of cadmium ion on the colony morphogenesis were reversible in heredity. The maximum absorption wavelength of crude red pigment extraction solution is 503 nm. The production of the red pigment reaches maximum capacity under 0.3% of CMC-Na added to the Czapek-Dox Medium solution. The results of chemical and physical stability experiments shows that the crude red pigment extraction solution is relatively stable under temperate range from 20˚C to 60˚C, the 0.002 g/L of sodium sul-phite has hyperchromic effect on the crude red pigment extraction solution, the stability declines when hydrogen peroxide added into the crude red pigment extraction solution, and the stability declines when the salinity content of NaCl increases. The dyeing effect of crude red pigment solution on the protein and starch food materials was equivalent to that of the monascus pigment solution diluted by 100 times. The research result could provide reference to the development of microbial red pigment resources.