彝医火草治疗大鼠骨关节炎作用机制研究

The Mechanism of Action of Yi Medicine Leontopodium franchetii in the Treatment of Osteoarthritis in Rats

摘要: 目的：研究彝药火草对大鼠骨关节炎的作用及作用机制，为火草的开发及研究提供相关证据。方法：将60只雄性SD大鼠随机分为6组，每组10只，分别为空白对照组、模型组、阳性药物(扶他林乳膏3.0 g/kg)组、火草低、中、高剂量(1.2 g生药/kg、2.4 g生药/kg、4.8 g生药/kg)组，采用注射0.2 mL 6%木瓜蛋白酶溶液方法进行造模。造模成功后，各组使用相应药物进行干预4周。4周后肉眼观察其关节、毛发等外观情况；光镜下观察软骨组织HE染色病理切片；使用酶联免疫吸附法(ELISA)对大鼠软骨组织的TNF-α、IL-1β含量水平进行检测；采用免疫组化法对大鼠软骨组织的MMP13、SOX-9基因表达水平进行检测。结果：与空白组比较，模型组的关节红肿严重，大鼠滑膜组织中炎症因子IL-1β、TNF-α含量显著上升(P < 0.01)，软骨组织中MMP13基因的表达量显著上升(P < 0.01)，SOX-9基因的表达量显著下降(P < 0.05)；与模型组相比，火草各剂量组大鼠滑膜组织中TNF-α含量及软骨组织中MMP13基因表达水平均显著下降(P < 0.01)，火草低、高剂量组滑膜组织中IL-1β含量显著下降(P < 0.05)，火草高剂量组软骨组织中SOX-9基因表达水平显著上升(P < 0.05)，其余含量差异无统计学意义。结论：外敷火草药物对大鼠软骨组织有着保护关节的作用，并对软骨组织有一定的修复功能，可缓解骨关节炎的病症。

Abstract: Objective: To study the effect and mechanism of tiacon of Yi medicine L. franchetii on osteoarthritis in rats, and to provide relevant evidence for the development and research of Fireweed. Methods: 60 male SD rats were randomly divided into 6 groups of 10 rats each, namely, the blank control group, the model group, the positive drug (3.0 g/kg of Voltaline cream) group, and the L. franchetii low, medium, and high dose (1.2 g of raw drug/kg, 2.4 g of raw drug/kg, and 4.8 g of raw drug/kg) groups, and were modeled by injecting 0.2 mL of 6% papain solution. Observe the appearance of joints and hairs with the naked eye after 4 weeks; observe the HE-stained pathological sections of cartilage tissues under light microscope; the levels of TNF-α and IL-1β in rat cartilage tissues were measured by enzyme-linked immunosorbent assay (ELISA); the levels of MMP13 and SOX-9 genes in rat cartilage tissues were measured by immunohistochemistry. Result: Compared with the blank group, the model group had severe joint redness and swelling, and the levels of inflammatory factors IL-1β and TNF-α, the expression of MMP13 gene increased significantly (P < 0.01), and the expression of SOX-9 gene decreased significantly (P < 0.05) in the synovial tissue of rats in each dose group of L. franchetii; compared with the model group, the TNF-α content and MMP13 gene expression level in the synovial tissue of rats in each dose group of L. franchetii were significantly decreased (P < 0.01), the IL-1β content in the low and high dose groups of L. franchetii was significantly decreased (P < 0.05), and theSOX-9 gene expression level in the high dose group of L. franchetii was significantly increased (P < 0.05), but the rest of the content differences were not statistically significant. Conclusion: The external application of L. franchetii drug has a joint-protective effect on the cartilage tissue of rats and has a certain repair function on the cartilage tissue, which can relieve the disease of osteoarthritis.