仪器分析综合实验设计：pH示差法和HPLC法测定黑米中总花青素含量

Comprehensive Experimental Design for Instrumental Analysis: Determination of Total Anthocyanins in Black Rice by pH Differential Method and HPLC Method

摘要: 设计了一个综合性实验，以矢车菊素-3-O-葡萄糖苷(C3G)作为外标物，旨在比较pH示差法和高效液相色谱法(HPLC)检测黑米中总花青素含量的结果，对两种方法的准确性进行评估。实验结果表明，pH示差法检测黑米中的C3G含量为29.95 mg/L，HPLC法测定结果为36.79 mg/L，两种检测结果差异较小。在缺乏高效液相色谱仪的实验室中，可用pH示差法替代检测黑米中总花青素含量。该综合实验将光谱、色谱知识内容与样品前处理、数据处理、问题分析等实际应用有机结合，通过科研全流程的模拟，增强了学生对理论知识与实际操作相互关系的理解，拓展了知识内容，激发了学习兴趣。学生通过该实验，巩固了《仪器分析》的基本原理和方法，掌握了系统科学的研究方法，对其专业素养与创新思维的提升与培养具有重要意义。

Abstract: A comprehensive experiment using centaurin-3-O-glucoside (C3G) as external standard was de-signed to compare the results of pH differential method and high performance liquid chromato-graphy (HPLC) for the determination of total anthocyanins in black rice, and to evaluate the accuracy of the two methods. The results showed that the content of C3G in black rice by pH difference method was 29.95 mg/L, and that by HPLC method was 36.79 mg/L. In the laboratory without high performance liquid chromatography, the pH differential method can be used to detect the total anthocyanins in black rice. The comprehensive experiment organically combined the knowledge content of spectrum and chromatography with practical applications such as sample pre-pro- cessing, data processing and problem analysis. Through the simulation of the whole process of scientific research, students’ understanding of the relationship between theoretical knowledge and practical operation was enhanced, knowledge content was expanded, and learning interest was stimulated. Through this experiment, students consolidated the basic principles and methods of Instrumental Analysis and mastered the research methods of systematic science, which was of great significance to the improvement and training of their professional quality and innovative thinking.