三种试剂对阪崎肠杆菌快速生化鉴定的比对研究

Comparative Study on Three Reagents for Rapid Biochemical Identification of Enterobacter sakazakii

摘要: 目的：比较三种阪崎肠杆菌生化鉴定试剂的可靠性；方法：以本实验室的传统生化鉴定管为基础，参考GB 4789.40-2016中的生化反应制备出干燥态生化鉴定条。以传统生化管(简称JDG)为参考，测试50株阪崎肠杆菌及其近源菌和分离株，比较本实验室自制HKEasyID阪崎肠杆菌生化鉴定条(简称HKEasyID)、A公司阪崎肠杆菌生化鉴定条(简称KitA)、B公司阪崎肠杆菌生化鉴定条(简称KitB)的鉴定效果。结果：对50株测试菌株的鉴定结果，HKEasyID、KitA和KitB三种试剂与JDG传统液态鉴定试剂盒鉴定结果相比平均符合率分别为98.67%、88.44%和90.22%，HKEasyID相比KitA和KitB，与JDG的符合率更高；HKEasyID、KitA、KitB和JDG四种鉴定试剂盒与标准要求相比平均符合率分别为97.11%、87.11%、89.11%和97.33%，HKEasyID和JDG相当，高于KitA和KitB。结论：HKEasyID鉴定条在操作性和符合率上优于A公司和B公司的鉴定条，在可靠性上与传统生化管接近，可作为常规生化鉴定试剂。

Abstract: Objective: To compare the reliability of three biochemical identification reagents for Enterobacter sakazakii. Method: Based on the traditional biochemical identification tube in our laboratory, a dry state biochemical identification strip was prepared by referring to the biochemical reaction in GB 4789.40-2016. Using the traditional biochemical tube (JDG) as a reference, 50 strains of E. sakazakii, their close source bacteria, and isolated strains were tested to compare the identification effects of our laboratory’s self-made HKEasyID E. sakazakii biochemical identification strip (HKEasyID), A company’s E. sakazakii biochemical identification strip (KitA), and B company’s E. sakazakii biochemical identification strip (KitB). Result: The identification results of 50 test strains showed that the average coincidence rates of HKEasyID, KitA and KitB with JDG traditional liquid identification kit were 98.67%, 88.44%, and 90.22%, respectively. HKEasyID had a higher coincidence rate with JDG compared to KitA and KitB; the average compliance rates of the four identification kits, HKHKEasyID, KitA, KitB, and JDG, compared to the standard requirements, were 97.11%, 87.11%, 89.11%, and 97.33%, respectively. HKEasyID and JDG were equivalent, higher than KitA and KitB. Conclusion: The HKEasyID identification strip is superior in operability and compliance to the identification strips of Company A and Company B, and is similar in reliability to traditional biochemical tubes. It can be used as a conventional biochemical identification reagent.