补骨脂不同炮制品对肾阳虚大鼠HPA轴的调控作用及肾毒性研究

Study on the Regulation of HPA Axis and Nephrotoxicity of Different Processed Products of Psoraleae Fructus Products in Rats with Kidney-Yang Deficiency

摘要: 目的：探究补骨脂不同炮制品对肾阳虚大鼠HPA轴的调控作用及肾毒性。方法：肌肉注射氢化可的松诱导肾阳虚模型，给药治疗15 d，ELISA法测定血清中的17-OHCS、CRH、ACTH、CORT含量，HE染色法观察肾上腺组织病变、形态；ELISA法测定血清及24 h尿液中Kim-1含量，HE染色法观察肾脏组织病理形态。结果：与模型组相比，补骨脂炮制品各组大鼠体重均增加，但无显著性差异(P > 0.05)；补骨脂炮制品各组大鼠肝指数、血清中的17-OHCS、CORT水平均高于模型组(P < 0.05)，肾上腺组织炎症病变得到不同程度的改善；酒制组的肾指数、肾上腺指数和盐炙组的肾指数显著高于模型组(P < 0.05)；盐炙组的肾上腺指数高于模型组，但无显著性差异(P > 0.05)；与空白组相比，补骨脂炮制品各组肾小管轻微扩张，酒制品病变较为严重；补骨脂生品组血液Kim-1水平显著高于空白组(P < 0.05)；补骨脂炮制品组中的血液、尿液Kim-1水平(除盐炙组血液Kim-1外)显著高于空白组(P < 0.05)。结论：补骨脂不同炮制品均能改善大鼠肾阳虚症状，调控HPA轴，但具体作用机制有一定差异；补骨脂不同炮制品仍保留有一定的肾毒性，但盐炙补骨脂毒性较低。

Abstract: Objective: To investigate the regulation of HPA axis and nephrotoxicity of different processed products of Psoraleae Fructus in rats with kidney-yang deficiency. Methods: Hydrocortisone was injected intramuscularly to create a model of kidney-yang deficiency, and the drug was administered for 15 d. ELISA was used to determine the content of 17-OHCS, CRH, ACTH, and CORT in serum, and HE staining was used to observe the adrenal tissue lesions and morphology; ELISA was used to determine the content of Kim-1 in serum and 24 h urine, and HE staining was used to observe the renal histopathology and morphology. Results: Compared with the model group, the body weight of rats in each group of different processed products of Psoraleae Fructus increased, but there was no significant difference (P > 0.05); the liver index and serum levels of 17-OHCS and CORT of the rats in each group of different processed products of Psoraleae Fructus were higher than those of the model group (P < 0.05), and the inflammatory lesions of the adrenal tissues were improved on different degrees; the renal index and adrenal index of the wine-made group and the renal index of salt-roasted group were significantly higher than those of the model group (P < 0.05); the adrenal index of the salt-roasted group was higher than that of the model group, but there was no significant difference (P > 0.05); compared with the blank group, the renal tubules of each group of different processed products of Psoraleae Fructus were slightly dilated, and the lesions of the wine product were more serious; the blood Kim-1 level of the tonic fat group was significantly higher than that of the blank group (P < 0.05); blood and urine Kim-1 levels (except for blood Kim-1 in the salt-roasted group) were significantly higher than those of the blank group in the processed products of Psoraleae Fructus (P < 0.05). Conclusion: Different processed products of Psoraleae Fructus can improve the symptoms of kidney-yang deficiency and regulate the HPA axis in rats, but the specific mechanism of action has some differences; different processed products of Psoraleae Fructus still retain a certain degree of nephrotoxicity, but the toxicity of salt-roasted tonic bone resin is lower.