探讨芦丁对急性软组织损伤的治疗作用

Exploring the Therapeutic Effects of Rutin on Acute Soft Tissue Injuries

摘要: 目的：本研究旨在探讨芦丁在急性软组织损伤治疗中的潜在作用。方法：分别使用人成纤维细胞(HFF-1)和成肌细胞(C2C12)通过划痕法和D-半乳糖法建立了体外损伤模型。实验分成三组：空白组，模型组(进行损伤造模)，以及芦丁组(芦丁预处理)。我们采用ELISA检测了不同组细胞上清液中IL-1β、IL-6和PGE-2的表达水平。此外，我们还评估了芦丁的细胞毒性和对细胞增殖活性的影响。结果：芦丁在浓度为10，20 μmol/L时对HFF-1和C2C12细胞的增殖活性无明显影响，而80 μmol/L的浓度明显抑制了细胞增殖。ELISA检测结果显示，相对于模型组，芦丁组降低了损伤细胞中IL-1β、IL-6和PGE-2的表达水平。结论：本研究表明，芦丁对急性软组织损伤具有潜在的治疗作用。芦丁能够降低损伤细胞中炎症因子的表达水平，减轻炎症反应，促进软组织的修复。

Abstract: Objective: This study aims to investigate the potential role of Rutin in the treatment of acute soft tissue injuries. Methods: In vitro injury models were established using human fibroblast cells (HFF-1) and myoblast cells (C2C12) through scratch and D-galactose methods, respectively. The ex-periments were divided into three groups: the blank group, the model group (injury modeling), and the Rutin group (Rutin pretreatment). ELISA was employed to detect the expression levels of IL-1β, IL-6, and PGE-2 in the supernatant of cells from different groups. Additionally, the cytotoxicity of Rutin and its impact on cell proliferation were assessed. Results: Rutin at concentrations of 10 and 20 μmol/L had no significant effect on the proliferation of HFF-1 and C2C12 cells, while a concentra-tion of 80 μmol/L significantly inhibited cell proliferation. ELISA results showed that, compared to the model group, the Rutin group reduced the expression levels of IL-1β, IL-6, and PGE-2 in injured cells. Conclusion: This study suggests that Rutin may have a potential therapeutic effect on acute soft tissue injuries. Rutin can reduce the expression levels of inflammatory factors in injured cells, alleviate the inflammatory response, and promote tissue repair.