多囊卵巢综合征患者卵巢颗粒细胞SF-1对芳香化酶P450的影响及其甲基化的调节机制

Effect of Granulosa Cell SF-1 on Aromatase P450 and Its Regulation of Methylation in Patients with PCOS

摘要: 目的：探讨多囊卵巢综合征患者卵巢颗粒细胞与正常对照组的SF-1mRNA表达及其甲基化差异性，并评价其临床意义。方法：随机选取2020年1月至2022年12月本院41例行IVF-ET的PCOS患者和72例因男方因素或输卵管因素行IVF-ET的对照组为研究对象，提取研究对象颗粒细胞，提取RNA，逆转录为cDNA进行荧光定量RT-PCR检测；行BSP法检测颗粒细胞DNA的甲基化水平。结果：与对照组卵巢颗粒细胞相比，PCOS患者CYP19和CYP19 PII的表达量显著降低(P < 0.05)，虽然LRH1和SF1在组间无显著差异，但是其与CYP19及CYP19 PII的变化呈显著正相关(P < 0.001)；SF-1基因在13个CpG位点在PCOS和对照组之间存在显著差异(P <0.05)；结论：该实验初步探讨了PCOS患者颗粒细胞芳香化酶P450活性下降机制，也在一定程度上解释了多囊患者雄激素升高的机制。

Abstract: Objective: To investigate the differences of SF-1 mRNA expression and methylation in granulosa cells of patients with polycystic ovary syndrome (PCOS) and normal control group, and to explore its clinical significance. Methods: A total of 41 PCOS patients who underwent IVF-ET were randomly se-lected from our hospital’s cases between January 2020 and December 2022, while the control group consisted of 72 cases undergoing IVF-ET due to male factor or tubal factor. Granulosa cells were ex-tracted from the study subjects for RNA extraction and reverse transcription into cDNA for fluores-cence quantitative RT-PCR; methylation levels were assessed using the line BSP method. Results: Compared to Granulocytes in normal patients, PCOS patients exhibited significantly lower expres-sion of CYP19 and CYP19 PII (P < 0.05), although LRH1 and SF1 did not show significant differences between groups; however, they demonstrated a significant positive correlation with changes in CYP19 and CYP19 PII (P < 0.001). The SF-1 gene was significantly different at 13 CpG sites between PCOS and healthy individuals (P < 0.05). Conclusions: This study initially investigated the mecha-nism of the decrease of aromatase P450 activity in granular cells of PCOS patients, and also ex-plained the mechanism of the increase of androgen in PCOS patients to a certain extent.