高压静电场对绿豆萌发和抗氧化生理影响的差异

Effects Comparison of the Germination and Antioxidant Physiology of Mung Bean Treated by High-Voltage Electrostatic Field

摘要: 为了探明高压静电场对绿豆(Vigna radiata L.)种子萌发生长调控的有效性及其对抗氧化生理的影响，研究了不同方向和强度高压静电场对绿豆种子萌发特性及其抗氧化特性影响的差异性。结果表明，同一强度(3 kV/m)高压正静电场处理对2个绿豆品种的发芽率、简化活力指数、芽长和根长都有明显的促进作用，相同强度的高压负静电场对2个绿豆品种的简化活力指数和芽长的生物学效应相反。不同方向高压静电场处理对2个绿豆品种鲜重的影响不明显。抗氧化生理研究显示，2个绿豆品种的SOD活性和含量出现峰值时的高压正静电场处理强度相同，小于12 kV/m高压正静电场处理可以诱导激活2个绿豆品种的SOD活性。2个绿豆品种萌发时的抗坏血酸含量(ASA)对不同强度高压正静电场处理表现不敏感，其萌发过程中H₂O₂的清除可能主要由CAT调控，而与APX无关。研究探讨了不同方向和强度高压静电场对绿豆种子萌发和抗氧化生理影响差异的原因。

Abstract: To clarify the effectiveness of high-voltage electrostatic field in regulating seed germination and growth, as well as their impact on antioxidant physiology, two mung beans cultivars (Vigna radiata L.) were used to compare the differences in germination characteristics and antioxidant properties. The results showed that the high-voltage positive electrostatic field at the same intensity (3 kV/m) significantly promoted the germination rate, simplified vigor index, shoot length and root length of the germinating seeds of two mung beans cultivars. However, the high-voltage negative electrostatic field with the same intensity had opposite effects on the simplified vigor index and shoot length. The influence of high-voltage electrostatic field with different directions on the fresh weight of the two mung beans was not significant. The antioxidant physiological study found that the SOD activity and content of the two mung bean varieties reached their peak under the same intensity of high-voltage positive electrostatic field. Additionally, the SOD activity of the two mung bean varie-ties could be stimulated by when the intensity of high-voltage positive electrostatic field was below12 kV/m. The contents of ascorbic acid (ASA) in the two mung bean cultivars were not sensi-tive to the high-voltage positive electrostatic fields with different intensities. The scavenging of H₂O₂ is mainly regulated by CAT, and is unrelated to APX during seed germination. The study discussed the reasons for the differences in the effects of high-voltage static electric fields with different direc-tions and intensities on mung bean seed germination and antioxidant physiology.