枸杞多糖通过生物节律基因Clock和Bmal1调节小鼠脂质代谢

Lycium Barbarum Polysaccharides Regulate Lipid Metabolism in Obese Mice through the Biological Rhythm Genes Clock and Bmal1

摘要: 目的：探究枸杞多糖通过调节生物节律基因(Clock, Bmal1)的变化来影响小鼠脂质代谢。方法：选用C57BL/6J雄性小鼠建立肥胖小鼠模型，不同剂量枸杞多糖进行干预。检测小鼠血清学指标总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)；利用苏木素伊红(HE)染色法进行肝脏组织形态学观察。利用蛋白免疫印记杂交(Western blot)检测Clock蛋白、Bmal1蛋白的表达水平。结果：血清学指标示：实验组TG、LDL及HDL水平与模型组相比均有显著性差异。HE染色结果显示：在肝脏的组织切片中，模型组中肝细胞出现大量的脂滴。枸杞多糖低剂量组、中剂量组和高剂量组肝细胞脂肪变的情况有不同程度改善。Western blot检测结果显示：在模型组中生物节律基因Clock和Bmal1的表达有所下降，在枸杞多糖干预组中Clock和Bmal1的表达有所上调。结论：枸杞多糖能够改善肥胖小鼠脂质的异常代谢，其机制可能是通过调节小鼠生物节律基因Clock和Bmal1的表达水平来实现的。

Abstract: Objective: To investigate the effect of LBP on lipid metabolism in mice by regulating changes in biorhythmic genes (Clock, Bmal1). Methods: C57BL/6J male mice were selected to establish an obese mouse model, and different doses of LBP were used for drug intervention. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were detected as serological indicators in mice. Liver histomorphometry was performed using hematoxylin eosin (HE) staining. The expression levels of Clock protein and Bmal1 protein were detected using Western blot (WB) hybridization for protein immunoblotting. Results: Serological indices showed that TG, LDL, HDL levels in the experimental group were all significantly different compared to the model group. The results of HE staining showed that in the tissue sections of the liver, a large number of lipid droplets appeared in the hepatocytes in the model group. Hepatocyte steatosis was improved to different degrees in the LBP low-dose, medium-dose and high-dose groups. The results of Western blot assay showed that the expression of biorhythm genes Clock and Bmal1 decreased in the model group, and the expression of Clock and Bmal1 was up-regulated in the LBP intervention group. Conclusion: LBP can improve the abnormal metabolism of lipids in obese mice, and its mechanism may be realized by regulating the expression levels of mouse biorhythm genes Clock and Bmal1.