单核细胞增生李斯特氏菌微量生化鉴定试剂盒应用效果研究

Study on the Application Effect of Microchemical Identification Kit for Listeria monocytogenes

摘要: 目的：评价本实验室研制的一步加样单核细胞增生李斯特氏菌微量生化鉴定试剂盒(简称EasyID)的可靠性和便捷性。方法：采用39株测试菌(目标菌25株和非目标菌14株)，对EasyID和其他两个厂家同种生化鉴定试剂盒(以下简称GS1、GS2)及传统管状生化鉴定套盒(以下简称CTG)进行测试，以GB4789.30-2016中提供的标准生化反应为参考。结果：在同等条件下，25株目标菌单核细胞增生李斯特氏菌在EasyID和GS1培养24 h，各项生化反应结果与标准反应符合率均为100%，但GS2和CTG需延长培养至48 h各项生化反应结果与标准反应符合率才达到100%；14株非目标在EasyID和GS1培养24 h，各项生化反应结果与标准反应符合率均为100%，但GS1、GS2和CTG需延长至48 h各项生化反应结果与标准反应符合率才达到100%。结论：EasyID操作便捷、反应速度和准确性优于GS1、GS2和CTG，因而可替代传统管状生化鉴定套盒应用于食源性致病菌单核细胞增生李斯特氏菌生化鉴定中。

Abstract: Objective: To evaluate the reliability and convenience of EasyID, a one-step sampling kit for the identification of Listeria monocytogenes. Methods: 39 strains (25 target strains, 14 non-target strains) were tested, EasyID and two other biochemical test kits (GS1, GS2) and the traditional tubular biochemical test kit (CTG) were tested, and the standard biochemical reaction provided in GB4789.30-2016 was taken as reference. Results: Under the same culture condition, all the biochemical reaction results of 25 target strains of Listeria monocytogenes in EasyID and GS1 for 24 hours were in accordance with the standard reaction rate of 100%, however, GS2 and CTG had to be cultured for 48 h before the coincidence rate of all biochemical reaction results with standard reaction reached 100%, and the coincidence rate of all biochemical reaction results with standard reaction was 100% for 14 non-target strains cultured for 24 h in EasyID and GS1, however, GS1, GS2 and CTG need to be extended to 48 hours before the agreement rate of all biochemical reaction results with standard reaction can reach 100%. Conclusion: EasyID is easier to operate, faster and more accurate than GS1, GS2 and CTG. Therefore, it can be used in the biochemical identification of food borne pathogenic bacteria Listeria monocytogenes instead of the traditional tubular biochemical identification kit.