基于网络药理学探讨栀子生姜豉汤治疗心脏神经官能症作用机制

Exploring the Mechanism of Action of Gardenia, Ginger and Black Bean Soup in the Treatment of Cardiac Neurosis Based on Network Pharmacology

摘要: 目的：利用网络药理学探究栀子生姜豉汤治疗心脏神经官能症(Cardiac neurosis, CN)的作用机制。方法：利用TCMSP检测栀子生姜豉汤的活性成分；通过GeneCards、OMIM数据库获取与CN相关的靶点；取栀子生姜豉汤与CN交集靶点；运用Cytoscape软件建立“药物–活性成分–靶点”的调控网络；运用STRING数据库构建蛋白质互作(PPI)网络图；利用Metascape平台进行GO和KEGG富集分析。结果：栀子生姜豉汤治疗CN的主要活性成分包括槲皮素、β-谷甾醇、山柰酚、豆甾醇等。核心靶点有TP53、AKT1、ESR1、MAPK1、IL6、TNF、IL1B、EGFR、CASP3等。KEGG分析主要涉及脂质和动脉粥样硬化通路、糖尿病并发症中的AGE-RAGE信号通路、甲状腺激素信号通路通路、EGFR酪氨酸激酶抑制剂耐药性通路等184条信号通路。结论：栀子生姜豉汤通过多成分、多靶点、多通路治疗心脏神经官能症，为临床的CN治疗及新药的研究提供了新的思路。

Abstract: Objective: To investigate the mechanism of action of Gardenia Sangjang Dangdang Tang in the treatment of Cardiac neurosis (CN) using network pharmacology. METHODS: The active ingredients of Gardenia Sangjang Dangdang Tang were detected by TCMSP; the targets related to CN were obtained by GeneCards and OMIM database; the intersection targets of Gardenia Sangjang Dangdang Tang and CN were taken; the regulatory network of “drug-active ingredient-target” was established by Cytoscape software; and protein interactions (PPI) were constructed by STRING database; the PPIs were also investigated by network pharmacology. STRING database was used to construct the protein-protein interaction (PPI) network map; Metascape platform was used for GO and KEGG enrichment analysis. RESULTS: The main active ingredients of Gardenia, Ginger, and Black Bean Soup for CN include quercetin, β-sitosterol, kaempferol, and stigmasterol. The core targets were TP53, AKT1, ESR1, MAPK1, IL6, TNF, IL1B, EGFR, CASP3, etc. KEGG analysis was mainly involved in 184 signaling pathways such as lipid and atherosclerosis pathway, AGE-RAGE signaling pathway in diabetic complications, thyroid hormone signaling pathway pathway, and EGFR tyrosine kinase inhibitor resistance pathway. Conclusion: Gardenia, ginger, and black bean soup provides new ideas for clinical CN treatment and research of new drugs through multi-component, multi-target, and multi-pathway treatment of cardiac neurosis.