健神利水方对脑出血大鼠p38 MAPK表达的影响

Effect of Jianshen Lishui Decoction on p38 MAPK Expression in Rats with Cerebral Hemorrhage

摘要: 目的：探究健神利水方对脑出血大鼠p38丝裂原活化蛋白激酶(p38 Mitogen-Activated Protein Kinase, p38 MAPK)表达的影响。方法：将50只SD大鼠随机分为假手术组(n = 10)和脑出血模型组(n = 40)，采用胶原酶制作脑出血大鼠模型，假手术组以等量生理盐水代替胶原酶造模；造模成功后，脑出血模型组随机均分为脑出血组(n = 10)、健神利水方低剂量组(n = 10)、健神利水方中剂量组(n = 10)及健神利水方高剂量组(n = 10) 4个组。其中，假手术组和脑出血组以等量生理盐水灌胃，健神利水方低、中、高剂量组分别以等量低、中、高剂量健神利水方灌胃，连续灌胃7天；采用Longa评分法对大鼠的神经功能进行评分；取大鼠脑组织，采用蛋白免疫印迹法(Western Blot, WB)检测p38 MAPK表达，采用反转录聚合酶链式反应(RT-PCR)检测大鼠脑组织p38 MAPK的mRNA表达水平。最后用统计软件SPSS 25.0对数据进行统计分析。结果：脑出血组较假手术组Longa评分、p38 MAPK、p38 MAPK的mRNA表达水平均显著升高(均P < 0.01)；低剂量组较脑出血组Longa评分、p38 MAPK、p38 MAPK的mRNA表达水平均升高(均P < 0.05)；中剂量组较低剂量组p38 MAPK、p38 MAPK的mRNA表达水平均升高(均P < 0.05)；高剂量组较中剂量组p38 MAPK、p38 MAPK的mRNA表达水平均升高(均P < 0.05)。结论：健神利水方能有效改善脑出血大鼠的神经损伤症状，其作用机制与抑制p38 MAPK的表达相关。

Abstract: Objective: To explore the effect of Jianshen Lishui decoction on the expression of p38 Mitogen-Activated Protein Kinase (p38 MAPK) in rats with cerebral hemorrhage. Methods: Fifty SD rats were randomly divided into sham operation group (n = 10) and cerebral hemorrhage model group (n = 40). The cerebral hemorrhage rat model was made with collagenase, and the sham operation group was made with the same amount of normal saline instead of collagenase. After the successful modeling, the cerebral hemorrhage model group was randomly divided into 4 groups: cerebral hemorrhage group (n = 10), Jianshen Lishui decoction prescription low-dose group (n = 10), Jianshen Lishui decoction prescription medium-dose group (n = 10) and Jianshen Lishui decoction prescription high-dose group (n = 10). Among them, sham operation group and cerebral hemorrhage group were given the same amount of normal saline, while Jianshen Lishui decoction prescription low-, medium- and high-dose groups were given the same amount of low-, medium- and high-dose of Jianshen Lishui decoction prescription, respectively, for continuous 7 days. The neural function of rats was evaluated by Longa score. The expression of p38 MAPK in rat brain tissues was detected by Western Blot (WB), and the mRNA expression level of p38 MAPK in rat brain tissues was detected by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Finally, statistical software SPSS 25.0 was used to analyze the data. Results: Compared with sham operation group, Longa score, p38 MAPK and p38 MAPK mRNA expression levels in cerebral hemorrhage group were significantly increased (all P < 0.01). Compared with cerebral hemorrhage group, Longa score, p38 MAPK and p38 MAPK mRNA expression levels in low-dose group were increased (all P < 0.05). The mRNA expression levels of p38 MAPK and p38 MAPK in medium dose group were increased compared with those in low dose group (all P < 0.05). The mRNA expression levels of p38 MAPK and p38 MAPK in high-dose group were higher than those in medium-dose group (all P < 0.05). Conclusion: Jianshen Lishui decoction can effectively improve the symptoms of nerve injury in rats with cerebral hemorrhage, and its mechanism is related to inhibiting the expression of p38 MAPK.