MCM7和TACSTD2在甲状腺乳头状癌中的表达及意义

Expression and Significance of MCM7 and TACSTD2 in Papillary Thyroid Carcinoma

摘要: 目的：研究微小染色体维持蛋白7 (MCM7)和肿瘤相关钙信号转导蛋白2 (TACSTD2)在甲状腺乳头状癌(PTC)中的表达及临床意义。方法：采用免疫组化方法对109例甲状腺良恶性病变(甲状腺乳头状癌69例、结节性甲状腺肿组织40例、癌旁甲状腺组织69例)中MCM7和TACSTD2的表达进行检测。结果：MCM7在上述组织中的阳性表达率分别为97.1% (67/69)、65.0% (26/40)和15.94% (11/69)，TACSTD2在上述组织的阳性表达率分别为98.55% (68/69)、60.0% (24/40)和17.39% (12/69)。两种蛋白在上述三种组织的阳性表达率差异均具有统计学意义(c² = 94.5 P < 0.001; c² = 93.6, P < 0.001)。卡方检验Bonferroni法两种蛋白在三组之间两两比较有统计学差异。相关性分析发现MCM7与TACSTD2的斯皮尔曼相关系数rs为0.383，P < 0.01，存在统计学意义。有序多分类logistic回归分析提示TACSTD2在甲状腺乳头状癌中表达阳性程度与肿瘤直径有统计学意义(P = 0.016, < 0.05)。结论：MCM7和TACSTD2在PTC组织中存在高表达，且TACSTD2的阳性表达与肿瘤直径呈正相关，可能参与肿瘤的发生发展；二者可能为诊断PTC提供新的分子生物学指标。

Abstract: Objective: To investigate the expression and clinical significance of minosomal maintenance protein 7 (MCM7) and tumor-associated calcium signal transducer 2 (TACSTD2) in papillary thyroid carcinoma (PTC). Methods: Immunohistochemistry was used to detect the expression of MCM7 and TACSTD2 in 109 cases of benign and malignant thyroid lesions (69 cases of papillary thyroid carcinoma, 40 cases of nodular goiter and 69 cases of adjacent thyroid tissues). Results: The positive expression rates of MCM7 in the above tissues were 97.1% (67/69), 65.0% (26/40) and 15.94% (11/69), respectively. The positive expression rates of TACSTD2 in the above tissues were 98.55% (68/69), 60.0% (24/40) and 17.39% (12/69), respectively. The positive expression rates of the two proteins in the above three tissues were statistically significant (c² = 94.5, P < 0.001; c² = 93.6, P < 0.001). Chi-square test and Bonferroni method showed that the two proteins were statistically different among the three groups. Correlation analysis showed that the Spearman correlation coefficient between MCM7 and TACSTD2 was 0.383, P < 0.01, which was statistically significant. Ordinal logistic regression analysis showed that the positive expression degree of TACSTD2 in papillary thyroid carcinoma was significantly correlated with tumor diameter (P = 0.016, < 0.05). Conclusions: MCM7 and TACSTD2 are highly expressed in PTC tissues, and the positive expression of TACSTD2 is positively correlated with tumor diameter, which may be involved in the occurrence and development of PTC. Both of them may provide new molecular biological markers for the diagnosis of PTC.