基于HPLC特征图谱的蜡梅花与其易混淆品梅花的鉴别研究

Identification of Flos Chimonanthi and Its Confused Product Mume Flos Based on HPLC Characteristic Chromatograms

摘要: 目的：建立蜡梅花药材的HPLC特征图谱，并对蜡梅花与梅花药材进行鉴别。方法：采用了Waters Cortecs T3 (100 mm × 2.1 mm, 1.6 μm)色谱柱，检测波长为355 nm，流动相为甲醇-0.2%磷酸的梯度洗脱，柱温为35℃，流速为每分钟0.3 mL，建立了10批次蜡梅花药材的特征图谱，并进行了相似度分析。同时对5批梅花药材进行特征图谱的检测，并进行了相似度分析。结果：建立了蜡梅花的HPLC特征图谱，并且方法学考察符合要求。通过使用该方法对蜡梅花与其易混淆品梅花进行特征图谱检测，两种药材存在明显差异，10批蜡梅花药材相似度均大于0.90，5批梅花药材相似度均小于0.60。结论：采用HPLC特征图谱可有效鉴别蜡梅花与其易混淆品梅花，本研究为蜡梅花的质量评价提供了有效方法。

Abstract: Objective: To establish HPLC characteristic chromatograms for the identification of Flos Chimonanthi, for the identification of Flos Chimonanthi and Mume Flos. Methods: The column was Waters Cortecs T3 (100 mm × 2.1 mm, 1.6 μm). The detection wavelength was 355 nm. The mobile phase was methanol-0.2% phosphoric acid in gradient elution with a flow rate of 0.3 mL/min. The column temperature was 35˚C. The characteristic chromatograms of 10 batches of Flos Chimonanthi were established, and the similarity was analyzed. The characteristic chromatograms of 5 batches of Mume Flos were detected, and the similarity was analyzed. Results: The HPLC characteristic chromatograms of Flos Chimonanthi were established. Methodological investigation met the requirements. By using this method to detect the characteristic chromatograms of Flos Chimonanthi and its confused product Mume Flos, there are significant differences between the two medicinal materials. The similarity of 10 batches of Flos Chimonanthi is greater than 0.90, and the similarity of 5 batches of Mume Flos is less than 0.60. Conclusion: Flos Chimonanthi and Mume Flos can be identified by HPLC characteristic chromatograms, the study can provide an effective method for quality evaluation of Flos Chimonanthi.